Present address: Laboratoire de Physiologie et de Génétique Moléculaire des Plantes, Université Libre de Bruxelles, CP 242, Boulevard du Triomphe, B-1050 Bruxelles, Belgium.
At-HSP17.6A, encoding a small heat-shock protein in Arabidopsis, can enhance osmotolerance upon overexpression
Article first published online: 23 DEC 2001
The Plant Journal
Volume 27, Issue 5, pages 407–415, September 2001
How to Cite
Sun, W., Bernard, C., Van De Cotte, B., Van Montagu, M. and Verbruggen, N. (2001), At-HSP17.6A, encoding a small heat-shock protein in Arabidopsis, can enhance osmotolerance upon overexpression. The Plant Journal, 27: 407–415. doi: 10.1046/j.1365-313X.2001.01107.x
- Issue published online: 23 DEC 2001
- Article first published online: 23 DEC 2001
- Received 27 March 2001; revised 11 June 2001; accepted 13 June 2001.
- molecular chaperone;
- salt stress;
- seed desiccation;
- transgenic plants
Owing to their sessile lifestyle, it is crucial for plants to acquire stress tolerance. The function of heat-shock proteins, including small heat-shock proteins (smHSPs), in stress tolerance is not fully explored. To gain further knowledge about the smHSPs, the gene that encoded the cytosolic class II smHSP in Arabidopsis thaliana (At-HSP17.6A) was characterized. The At-HSP17.6A expression was induced by heat and osmotic stress, as well as during seed development. Accumulation of At-HSP17.6A proteins could be detected with heat and at a late stage of seed development, but not with osmotic stress, suggesting stress-induced post-transcriptional regulation of At-HSP17.6A expression. Overproduction of At-HSP17.6A could increase salt and drought tolerance in Arabidopsis. The chaperone activity of At-HSP17.6A was demonstrated in vitro.