Present address: Eastern Cereal and Oilseeds Research Center, Agriculture and Agri-Food Canada, Ottawa, ON KIA 0C6, Canada.
Regulation of plant water loss by manipulating the expression of phospholipase Dα
Article first published online: 23 DEC 2001
The Plant Journal
Volume 28, Issue 2, pages 135–144, October 2001
How to Cite
Sang, Y., Zheng, S., Li, W., Huang, B. and Wang, X. (2001), Regulation of plant water loss by manipulating the expression of phospholipase Dα. The Plant Journal, 28: 135–144. doi: 10.1046/j.1365-313X.2001.01138.x
- Issue published online: 23 DEC 2001
- Article first published online: 23 DEC 2001
- Received 9 May 2001; revised 4 July 2001; accepted 9 July 2001.
- abscisic acid;
- signal transduction;
- stress response
Phospholipase D (PLD) has been implicated in various processes, including signal transduction, membrane trafficking, and membrane degradation. Multiple forms of PLD with distinct biochemical properties have been described in the cell. In Arabidopsis, PLDα and PLDγ, but not PLDβ, were detected in guard cells, and antisense suppression resulted in a specific loss of PLDα. The abrogation of PLDα rendered plants less sensitive to abscisic acid and impaired stomatal closure induced by water deficits. PLDα-depleted plants exhibited accelerated transpirational water loss and a decreased ability to tolerate drought stress. Overexpression of PLDα enhanced the leaf's sensitivity to abscisic acid. These findings provide molecular and physiological evidence that PLDα plays a crucial role in regulating stomatal movement and plant-water status.