Distinct cell-specific expression patterns of early and late gibberellin biosynthetic genes during Arabidopsis seed germination

Authors

  • Shinjiro Yamaguchi,

    Corresponding author
    1. Developmental, Cell and Molecular Biology Group, Department of Biology, Duke University, Box 91000, Durham, North Carolina 27708–1000, USA, and
    2. Plant Science Center, The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2–1, Wako-shi, 351–0198, Japan
      For correspondence (fax +81 48 462 4691; e-mail: shinjiro@postman.riken.go.jp
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  • Yuji Kamiya,

    1. Plant Science Center, The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2–1, Wako-shi, 351–0198, Japan
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  • Tai-ping Sun

    1. Developmental, Cell and Molecular Biology Group, Department of Biology, Duke University, Box 91000, Durham, North Carolina 27708–1000, USA, and
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For correspondence (fax +81 48 462 4691; e-mail: shinjiro@postman.riken.go.jp).

Summary

Gibberellins (GAs) are biosynthesized through a complex pathway that involves several classes of enzymes. To predict sites of individual GA biosynthetic steps, we studied cell type-specific expression of genes encoding early and late GA biosynthetic enzymes in germinating Arabidopsis seeds. We showed that expression of two genes, AtGA3ox1 and AtGA3ox2, encoding GA 3-oxidase, which catalyzes the terminal biosynthetic step, was mainly localized in the cortex and endodermis of embryo axes in germinating seeds. Because another GA biosynthetic gene, AtKO1, coding for ent-kaurene oxidase, exhibited a similar cell-specific expression pattern, we predicted that the synthesis of bioactive GAs from ent-kaurene oxidation occurs in the same cell types during seed germination. We also showed that the cortical cells expand during germination, suggesting a spatial correlation between GA production and response. However, promoter activity of the AtCPS1 gene, responsible for the first committed step in GA biosynthesis, was detected exclusively in the embryo provasculature in germinating seeds. When the AtCPS1 cDNA was expressed only in the cortex and endodermis of non-germinating ga1–3 seeds (deficient in AtCPS1) using the AtGA3ox2 promoter, germination was not as resistant to a GA biosynthesis inhibitor as expression in the provasculature. These results suggest that the biosynthesis of GAs during seed germination takes place in two separate locations with the early step occurring in the provasculature and the later steps in the cortex and endodermis. This implies that intercellular transport of an intermediate of the GA biosynthetic pathway is required to produce bioactive GAs.

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