The broad-spectrum potato cyst nematode resistance gene (Hero) from tomato is the only member of a large gene family of NBS-LRR genes with an unusual amino acid repeat in the LRR region

Authors

  • Karin Ernst,

    Corresponding author
    1. Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D-06466 Gatersleben, Germany
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    • Present address: Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany

  • Amar Kumar,

    1. Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK
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  • Doris Kriseleit,

    1. Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D-06466 Gatersleben, Germany
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  • Dorothee-U. Kloos,

    1. Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D-06466 Gatersleben, Germany
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    • Present address: BIOBASE GmbH, Maschroder Weg 1b, 38124 Braunschweig, Germany

  • Mark S. Phillips,

    1. Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK
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  • Martin W. Ganal

    1. Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D-06466 Gatersleben, Germany
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    • Present address: Trait Genetics GmbH, Am Schwabeplan 1b, D-06466 Gatersleben, Germany


*For correspondence (fax 49 221 811 4871; e-mail karin.ernst@rz.uni-duesseldorf.de).

Summary

The Hero gene of tomato is a broad spectrum resistance gene that confers a high level of resistance to all pathotypes of the potato cyst nematodes Globodera rostochiensis and partial resistance to G. pallida. The gene was identified by map-based cloning, sequencing and complementation analysis of two susceptible tomato lines with an array of 13 overlapping cosmids spanning a total distance of 135 kb. Hero encodes a protein with a nucleotide-binding site (NBS) and a leucine-rich-repeat (LRR) domain and is a member of a gene family of 14 highly homologous genes, which are clustered within a continuous 118-kb region. The isolated Hero gene displayed resistance to various G. rostochiensis pathotypes and partial resistance to G. pallida pathotype Pa2/3 in transgenic tomato lines. None of the Hero homologues conferred resistance to G. rostochiensis pathotypes. Hero can be distinguished from its homologues by the length of a compound hexanucleotide microsatellite, which codes for a charged and repetitive amino acid domain within the LRR. We propose that the expansion of this microsatellite may be involved in the evolution of the Hero resistance gene.

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