The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells
Article first published online: 17 JUL 2002
The Plant Journal
Volume 31, Issue 2, pages 137–147, July 2002
How to Cite
Simon-Plas, F., Elmayan, T. and Blein, J.-P. (2002), The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells. The Plant Journal, 31: 137–147. doi: 10.1046/j.1365-313X.2002.01342.x
- Issue published online: 17 JUL 2002
- Article first published online: 17 JUL 2002
- Received 8 March 2002; accepted 22 March 2002.
- active oxygen species;
- NADPH oxidase;
- plasma membrane;
A cDNA encoding a protein, NtrbohD, located on the plasma membrane and homologue to the flavocytochrome of the neutrophil NADPH oxidase, was cloned in tobacco. The corresponding mRNA was accumulated when tobacco leaves and cells were treated with the fungal elicitor cryptogein. After elicitation with cryptogein, tobacco cells transformed with antisense constructs of NtrbohD showed the same extracellular alkalinization as the control, but no longer produced active oxygen species (AOS). This work represents the first demonstration of the function of a homologue of gp91–phox in AOS production in elicited tobacco cells.