In planta protein–protein interactions assessed using a nanovirus-based replication and expression system
Article first published online: 5 SEP 2002
The Plant Journal
Volume 31, Issue 6, pages 767–775, September 2002
How to Cite
Aronson, M. N., Complainville, A., Clérot, D., Alcalde, H., Katul, L., Vetten, H. J., Gronenborn, B. and Timchenko, T. (2002), In planta protein–protein interactions assessed using a nanovirus-based replication and expression system. The Plant Journal, 31: 767–775. doi: 10.1046/j.1365-313X.2002.01388.x
- Issue published online: 5 SEP 2002
- Article first published online: 5 SEP 2002
- Received 22 March 2002; revised 13 May 2002; accepted 17 May 2002.
The multipartite genome of the nanovirus Faba bean necrotic yellows virus, which consists of one gene on each DNA component, was exploited to construct a series of virus-based episomal vectors designed for transient replication and gene expression in plants. This nanovirus based expression system yields high levels of protein which allows isolation of recombinant protein and protein complexes from plant tissues. As examples, we demonstrated in planta interaction between the nanovirus F-box protein Clink and SKP1, a constituent of the ubiquitin-dependent protein turnover pathway. Thus, replicative nanovirus vectors provide a simple and efficient means for in planta characterization of protein-protein interaction.