Overexpression of Bax inhibitor suppresses the fungal elicitor-induced cell death in rice (Oryza sativa L.) cells
Article first published online: 6 FEB 2003
The Plant Journal
Volume 33, Issue 3, pages 425–434, February 2003
How to Cite
Matsumura, H., Nirasawa, S., Kiba, A., Urasaki, N., Saitoh, H., Ito, M., Kawai-Yamada, M., Uchimiya, H. and Terauchi, R. (2003), Overexpression of Bax inhibitor suppresses the fungal elicitor-induced cell death in rice (Oryza sativa L.) cells. The Plant Journal, 33: 425–434. doi: 10.1046/j.1365-313X.2003.01639.x
- Issue published online: 6 FEB 2003
- Article first published online: 6 FEB 2003
- Received 28 August 2002; revised 15 October 2002; accepted 29 October 2002.
- Bax inhibitor;
- cell death;
- Magnaporthe grisea
Treatment of suspension-cultured cells of rice (Oryza sativa L.) with cell wall extract of rice blast fungus (Magnaporthe grisea) elicits a rapid generation of H2O2, alkalinization of culture medium, and eventual cell death. To elucidate genes involved in these processes, we exploited SAGE (Serial Analysis of Gene Expression) technique for the molecular analysis of cell death in suspension-cultured cells treated with the elicitor. Among the downregulated genes in the elicitor-treated cells, a BI-1 gene coding for Bax inhibitor was identified. Transgenic rice cells overexpressing Arabidopsis BI-1 gene showed sustainable cell survival when challenged with M. grisea elicitor. Thus, the plant Bax inhibitor plays a functional role in regulating cell death in the rice cell culture system.