Disruption of Arabidopsis thaliana MYB26 results in male sterility due to non-dehiscent anthers

Authors

  • Sabine Steiner-Lange,

    Corresponding author
    1. Zentrum zur Identifizierung von Genfunktionen durch Insertionsmutagenese in Arabidopsis thaliana (ZIGIA), Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Köln, Germany,
      For correspondence (fax +49 221 5062361; e-mail steiner@mpiz-koeln.mpg.de).
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  • Ulrike S. Unte,

    1. Zentrum zur Identifizierung von Genfunktionen durch Insertionsmutagenese in Arabidopsis thaliana (ZIGIA), Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Köln, Germany,
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  • Luca Eckstein,

    1. Zentrum zur Identifizierung von Genfunktionen durch Insertionsmutagenese in Arabidopsis thaliana (ZIGIA), Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Köln, Germany,
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  • Caiyun Yang,

    1. School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leics LE12 5RD, UK,
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  • Zoe A. Wilson,

    1. School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leics LE12 5RD, UK,
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  • Elmon Schmelzer,

    1. CeMic, Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Köln, Germany, and
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  • Koen Dekker,

    1. Zentrum zur Identifizierung von Genfunktionen durch Insertionsmutagenese in Arabidopsis thaliana (ZIGIA), Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Köln, Germany,
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  • Heinz Saedler

    1. Abteilung Molekulare Pflanzengenetik, Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Köln, Germany
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For correspondence (fax +49 221 5062361; e-mail steiner@mpiz-koeln.mpg.de).

Summary

A male sterile mutant with a defect in anther dehiscence was identified in an Arabidopsis thaliana population mutagenized with the Zea mays transposon En-1/Spm. Mutants produce viable pollen that can fertilize when released mechanically from the anthers. Mutant stamens are of normal size and shape, but lack cell wall fortifications in the endothecial cell layer of the anther, which are required for the dehiscence process. The mutant phenotype was shown to be caused by a transposon insertion in AtMYB26, disrupting the putative DNA-binding domain of this R2R3-type MYB transcription factor. RT-PCR revealed that expression of AtMYB26 is restricted to inflorescences. Sterility was shown to be stable under several environmental conditions. The high stability of the sterile phenotype, together with the fact that pollen is functional, makes AtMYB26 and its orthologs a valuable tool for manipulating male fertility in higher plants.

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