Monitoring expression profiles of Arabidopsis gene expression during rehydration process after dehydration using ca. 7000 full-length cDNA microarray

Authors

  • Youko Oono,

    1. Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
    2. Master's Program in Biosystem Studies, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-0074, Japan,
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  • Motoaki Seki,

    1. Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
    2. Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
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  • Tokihiko Nanjo,

    1. Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
    2. Genesis Research Institute, Inc., 4-1-35 Noritake-Shinmachi Nishi-ku, Nagoya, Aichi 451-0051, Japan,
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    • Current address: Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute (FFPRI), 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan.

  • Mari Narusaka,

    1. Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
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  • Miki Fujita,

    1. Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
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  • Rie Satoh,

    1. Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
    2. Biological Resources Division, Japan International Research Center for Agricultural Sciences, 2-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan, and
    3. Institute of Biological Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan
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  • Masakazu Satou,

    1. Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
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  • Tetsuya Sakurai,

    1. Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
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  • Junko Ishida,

    1. Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
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  • Kenji Akiyama,

    1. Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
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  • Kei Iida,

    1. Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
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  • Kyonoshin Maruyama,

    1. Biological Resources Division, Japan International Research Center for Agricultural Sciences, 2-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan, and
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  • Shinobu Satoh,

    1. Master's Program in Biosystem Studies, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-0074, Japan,
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  • Kazuko Yamaguchi-Shinozaki,

    1. Biological Resources Division, Japan International Research Center for Agricultural Sciences, 2-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan, and
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  • Kazuo Shinozaki

    Corresponding author
    1. Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
    2. Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
    3. Institute of Biological Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan
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For correspondence (fax +81 298 36 9060; e-mail sinozaki@rtc.riken.go.jp).

Summary

Plants respond and adapt to drought stress in order to survive under stress conditions. Several genes that respond to drought at the transcriptional level have been described, but there are few reports on genes involved in the recovery from dehydration. Analysis of rehydration-inducible genes should help not only to understand the molecular mechanisms of stress responses in higher plants, but also to improve the stress tolerance of crops by gene manipulation. We used a full-length cDNA microarray containing ca. 7000 Arabidopsis full-length cDNAs and identified 152 rehydration-inducible genes. Venn diagram analysis showed relationship of the rehydration-inducible genes to proline-inducible and water-treatment-inducible genes. Among the 152 rehydration-inducible genes, 58 genes contained the ACTCAT sequence involved in proline- and hypoosmolarity-inducible gene expression in their promoter regions, suggesting that ACTCAT sequence is a major cis-acting element involved in rehydration-inducible gene expression, and that some novel cis-acting elements are involved in rehydration-inducible gene expression. Functional analysis of rehydration-inducible and rehydration-repressed genes revealed their functions not only in the release from a stressed status but also in the recovery of growth in plants.

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