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Coordinate repression of regulators of embryonic identity by PICKLE during germination in Arabidopsis

  1. Stanley Dean Rider Jr1,
  2. James T. Henderson1,
  3. Ronald E. Jerome2,
  4. Howard J. Edenberg2,
  5. Jeanne Romero-Severson3,
  6. Joe Ogas1,*

Article first published online: 30 JUN 2003

DOI: 10.1046/j.1365-313X.2003.01783.x

Issue

The Plant Journal

The Plant Journal

Volume 35, Issue 1, pages 33–43, July 2003

Additional Information

How to Cite

Dean Rider, S., Henderson, J. T., Jerome, R. E., Edenberg, H. J., Romero-Severson, J. and Ogas, J. (2003), Coordinate repression of regulators of embryonic identity by PICKLE during germination in Arabidopsis. The Plant Journal, 35: 33–43. doi: 10.1046/j.1365-313X.2003.01783.x

Author Information

  1. 1

    Department of Biochemistry, Purdue University, West Lafayette, Indiana, IN 47907-2063, USA,

  2. 2

    Department of Biochemistry and Molecular Biology and Center for Medical Genomics, Indiana University School of Medicine, Indianapolis, Indiana, IN 46202, USA, and

  3. 3

    Department of Forestry and Natural Resources and Computational Genomics Center, Purdue University, West Lafayette, Indiana, IN 47907-1159, USA

* For correspondence (fax +1 765 494 7897; e-mail ogas@purdue.edu).

Publication History

  1. Issue published online: 30 JUN 2003
  2. Article first published online: 30 JUN 2003
  3. Received 10 February 2003; revised 20 March 2003; accepted 21 March 2003.

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Keywords:

  • CHD3;
  • chromatin-remodeling factors;
  • developmental transition;
  • embryo;
  • seed;
  • germination

Summary

In angiosperms, germination represents an important developmental transition during which embryonic identity is repressed and vegetative identity emerges. PICKLE (PKL) encodes a CHD3-chromatin-remodeling factor necessary for the repression of expression of LEAFY COTYLEDON1 (LEC1), a central regulator of embryogenesis. A candidate gene approach and microarray analysis identified nine additional genes that exhibit PKL-dependent repression of expression during germination. Transcripts for all three LEAFY COTYLEDON genes, LEC1, LEC2, and FUS3, exhibit PKL-dependent repression, and all three transcripts are elevated more than 100-fold in pkl primary roots that inappropriately express embryonic traits (pickle roots). Three other genes that exhibit PKL-dependent regulation have expression patterns correlated with zygotic or somatic embryogenesis, and one gene encodes a putative Lin-11, Isl-1, MEC-3 (LIM) domain transcriptional regulator that is preferentially expressed in siliques. Genes that exhibit PKL-dependent repression during germination are not necessarily regulated by PKL at other points in development. Our data suggest that PKL selectively regulates a suite of genes during germination to repress embryonic identity. In particular, we propose that PKL acts as a master regulator of the LEAFY COTYLEDON genes, and that joint derepression of these genes is likely to contribute substantially to expression of embryonic identity in pkl seedlings.

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