The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats

Authors

  • Gregory G. Brown,

    Corresponding author
    1. 1 Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1, and2 DNA LandMarks, Inc.#, St-Jean-sur-Richelieu, Quebec, Canada J3B 6X3
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  • 1,2, Nataša Formanová,

    1. 1 Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1, and2 DNA LandMarks, Inc.#, St-Jean-sur-Richelieu, Quebec, Canada J3B 6X3
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  • 1, Hua Jin,

    1. 1 Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1, and2 DNA LandMarks, Inc.#, St-Jean-sur-Richelieu, Quebec, Canada J3B 6X3
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  • 2, Richard Wargachuk,

    1. 1 Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1, and2 DNA LandMarks, Inc.#, St-Jean-sur-Richelieu, Quebec, Canada J3B 6X3
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  • 1 Charles Dendy,

    1. 1 Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1, and2 DNA LandMarks, Inc.#, St-Jean-sur-Richelieu, Quebec, Canada J3B 6X3
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  • 1, Prashant Patil,

    1. 1 Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1, and2 DNA LandMarks, Inc.#, St-Jean-sur-Richelieu, Quebec, Canada J3B 6X3
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  • 1,2 Martin Laforest,

    1. 1 Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1, and2 DNA LandMarks, Inc.#, St-Jean-sur-Richelieu, Quebec, Canada J3B 6X3
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  • 2 Jinfa Zhang,

    1. 1 Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1, and2 DNA LandMarks, Inc.#, St-Jean-sur-Richelieu, Quebec, Canada J3B 6X3
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  • 1, Wing Y. Cheung,

    1. 1 Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1, and2 DNA LandMarks, Inc.#, St-Jean-sur-Richelieu, Quebec, Canada J3B 6X3
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  • and 2 Benoit S. Landry 2

    1. 1 Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1, and2 DNA LandMarks, Inc.#, St-Jean-sur-Richelieu, Quebec, Canada J3B 6X3
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For correspondence (fax +1 450 358 1154; e-mail browng@dnalandmarks.ca).

Current address: Max Planck Institut fuer Chemische Oekologie, Abteilung fuer Genetik und Evolution, Winzerlaer Strasse 10, Jena D07745, Germany.

Current address: Performance Plants Inc., Biosciences Complex, c/o Queen's University, Kingston ON, Canada K7L 3N6.

Current address: Health Canada, 130 Colonnade Road, Nepean ON, Canada K1A 0K9.

Current address: Department of Agronomy and Horticulture, Skeen Hall, New Mexico State University, Las Cruces, NM 88003, USA.

DNA LandMarks, Inc. is a member of the BASF Plant Science Group.

Summary

A single radish nuclear gene, Rfo, restores Ogura (ogu) cytoplasmic male sterility (CMS) in Brassica napus. A map-based cloning approach relying on synteny between radish and Arabidopsis was used to clone Rfo. A radish gene encoding a 687-amino-acid protein with a predicted mitochondrial targeting pre-sequence was found to confer male fertility upon transformation into ogu CMS B. napus. This gene, like the recently described Petunia Rf gene, codes for a pentatricopeptide repeat (PPR)-containing protein with multiple, in this case 16, PPR domains. Two similar genes that do not appear to function as Rfo flank this gene. Comparison of the Rfo region with the syntenic Arabidopsis region indicates that a PPR gene is not present at the Rfo-equivalent site in Arabidopsis, although a smaller and related PPR gene is found about 40 kb from this site. The implications of these findings for the evolution of restorer genes and other PPR encoding genes are discussed.

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