Transcriptome analysis of Arabidopsis colonized by a plant-growth promoting rhizobacterium reveals a general effect on disease resistance

Authors

  • Fabienne Cartieaux,

    1. Laboratoire de Biologie du Développement des Plantes, UMR CNRS-CEA-University méditerranée 163, DSV.DEVM, CEA/Cadarache, F-13108 Saint Paul lez Durance Cedex, France,
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  • Marie-Christine Thibaud,

    1. Laboratoire de Biologie du Développement des Plantes, UMR CNRS-CEA-University méditerranée 163, DSV.DEVM, CEA/Cadarache, F-13108 Saint Paul lez Durance Cedex, France,
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  • Laurent Zimmerli,

    1. Department of Plant Biology, Carnegie Institution, Stanford, CA 94305, USA, and
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  • Philippe Lessard,

    1. Laboratoire de Biologie du Développement des Plantes, UMR CNRS-CEA-University méditerranée 163, DSV.DEVM, CEA/Cadarache, F-13108 Saint Paul lez Durance Cedex, France,
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  • Catherine Sarrobert,

    1. Laboratoire de Biologie du Développement des Plantes, UMR CNRS-CEA-University méditerranée 163, DSV.DEVM, CEA/Cadarache, F-13108 Saint Paul lez Durance Cedex, France,
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  • Pascale David,

    1. Laboratoire de Biologie du Développement des Plantes, UMR CNRS-CEA-University méditerranée 163, DSV.DEVM, CEA/Cadarache, F-13108 Saint Paul lez Durance Cedex, France,
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  • Alain Gerbaud,

    1. Laboratoire de Biologie du Développement des Plantes, UMR CNRS-CEA-University méditerranée 163, DSV.DEVM, CEA/Cadarache, F-13108 Saint Paul lez Durance Cedex, France,
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  • Christophe Robaglia,

    1. Laboratoire de Biologie du Développement des Plantes, UMR CNRS-CEA-University méditerranée 163, DSV.DEVM, CEA/Cadarache, F-13108 Saint Paul lez Durance Cedex, France,
    2. Laboratoire de Génétique et de Biophysique des Plantes, UMR CNRS-CEA-University méditerranée 163, DSV.DEVM, CEA/Cadarache, F-13108 Saint Paul lez Durance Cedex, France
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  • Shauna Somerville,

    1. Department of Plant Biology, Carnegie Institution, Stanford, CA 94305, USA, and
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  • Laurent Nussaume

    Corresponding author
    1. Laboratoire de Biologie du Développement des Plantes, UMR CNRS-CEA-University méditerranée 163, DSV.DEVM, CEA/Cadarache, F-13108 Saint Paul lez Durance Cedex, France,
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For correspondence (fax +33 442 25 46 56; e-mail lnussaume@cea.fr).

Present address: Laboratoire des Symbioses Tropicales et Méditerranéennes, UMR 1063, Campus International de Baillarguet, F-34398 Montpellier Cedex 5, France.

Present address: Biogemma S.A.S., Campus Universitaire des Cézaux, 24 Avenue des Landais, 63170 Aubière, France.

Present address: Systèmes agraires et développement, INRA, Route de Bois Maché, F-17450 Saint laurent de la Prée, France.

Both first authors have contributed equally to this work.

Summary

RNA transcript levels of Arabidopsis plants, infected by the rhizobacterium Pseudomonas thivervalensis (strain MLG45), and axenic control plants were compared using cDNA microarrays representing approximately 14 300 genes. The analysis revealed an increase of defence-related transcripts in the shoots of bacterized plants relative to control (axenic) plants. These modifications of transcript levels were confirmed by physiological experiments. Plants infected with P. thivervalensis were more resistant to subsequent infections by the virulent pathogen P. syringae pv. tomato (strain DC3000) than control plants. In addition, photosynthesis rates were repressed consistently with the reduced growth of plants colonized by P. thivervalensis. These results highlight the value of molecular phenotyping to predict physiological changes.

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