Conservation of class C function of floral organ development during 300 million years of evolution from gymnosperms to angiosperms
Article first published online: 14 AUG 2008
The Plant Journal
Volume 37, Issue 4, pages 566–577, February 2004
How to Cite
Zhang, P., Tan, H. T. W., Pwee, K.-H. and Kumar, P. P. (2004), Conservation of class C function of floral organ development during 300 million years of evolution from gymnosperms to angiosperms. The Plant Journal, 37: 566–577. doi: 10.1046/j.1365-313X.2003.01983.x
- Issue published online: 14 AUG 2008
- Article first published online: 14 AUG 2008
- Received 4 August 2003; revised 27 October 2003; accepted 11 November 2003.
Fig. S1. The full-length cDNA (upper row) and amino acid (lower row) sequences of CyAG gene. For the amino acid sequence of CyAG, the MADS-box is shown in bold letters, I-domain is shown by italic letters, K domain is boxed, and the rest is the C-terminal. For cDNA sequence, the translation stop codon is indicated by an asterisk, and the polyA signal (TATAA) is underlined. The primers (AG1F/AG1R, AG2F/AG2R, AG3F/AG3R, and AG4F/AG4R) designed for cloning the introns of CyAG gene are underlined with arrows indicating their directions. The primers AG4F/AG4R were used to synthesize the 3' CyAG-specific probe for genomic DNA and RNA blot analyses.
Fig. S2. Phylogenetic tree of plant MADS-box proteins (MIK region) in the AGAMOUS, DEFICIENS (DEF) and SQUAMOSA (SQUA) subfamilies. A single most parsimonious tree was obtained. Bootstrap values for 100 replicates that are above 50% are shown at the branches.
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