Changes in the cytoskeleton accompanying infection-induced nuclear movements and the hypersensitive response in plant cells invaded by rust fungi
Article first published online: 25 DEC 2001
The Plant Journal
Volume 16, Issue 2, pages 191–200, October 1998
How to Cite
Skalamera, D. and Heath, M. C. (1998), Changes in the cytoskeleton accompanying infection-induced nuclear movements and the hypersensitive response in plant cells invaded by rust fungi. The Plant Journal, 16: 191–200. doi: 10.1046/j.1365-313x.1998.00285.x
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
- Received 30 June 1998; revised 24 August 1998; accepted 27 August 1998.
During the infection of cowpea(Vigna unguiculata)by the cowpea rust fungus(Uromyces vignae,race 1)the plant nucleus moves towards and away from the invading hypha and eventually moves close to the fungus in the susceptible cultivar while it remains away in two cultivars which subsequently respond by resistance gene-dependent plant cell death (the hypersensitive response, HR). The role of plant cytoskeleton in these responses was investigated by fluorescent microscopy and treatments with anticytoskeletal drugs. Observations of microtubule organization prior to cell death revealed that the sequence of events leading to protoplast collapse differed between the two resistant cultivars, suggesting a possibility of multiple pathways for cellular degradation during the HR. Different fixations produced two different microfilament patterns: a filament network and cables. Microfilament network remained visible even at later stages of cell death. Oryzalin and taxol reduced the incidence of autofluorescence that develops late in the death process, indicating a role of microtubules in the deposition of phenolics by adjacent living cells. Cell death and nuclear movements were not affected by oryzalin and taxol but were inhibited by cytochalasin E, suggesting that the microfilaments are required for the HR.