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Floral dip: a simplified method forAgrobacterium-mediated transformation ofArabidopsis thaliana
Article first published online: 9 OCT 2008
DOI: 10.1046/j.1365-313x.1998.00343.x
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How to Cite
Clough, S. J. and Bent, A. F. (1998), Floral dip: a simplified method forAgrobacterium-mediated transformation ofArabidopsis thaliana. The Plant Journal, 16: 735–743. doi: 10.1046/j.1365-313x.1998.00343.x
Publication History
- Issue published online: 9 OCT 2008
- Article first published online: 9 OCT 2008
- Received 26 June 1998; revised 15 October 1998; accepted 26 October 1998.
References
- , , , , , , 1997Current Protocols in Molecular Biology. New York: John Wiley & Sons.
- & 1997 T-DNA insertion mutagenesis in Arabidopsis: going back and forth. Trends Genet., 13, 152 156.
- , , 1993 In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C. R. Acad. Sci. Paris, Life Sciences, 316, 1194 1199.
- & 1998 Agrobacterium germ-line transformation: transformation of Arabidopsis without tissue culture. In Plant Molecular Biology Manual ( Gelvin, S.B., ed..). Netherlands: Kluwer Academic Publishers. B7, 1 14.
- , , , , , , , 1994 RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. Science, 265, 1856 1860.
- , , 1993 A binary vector based on basta resistance for in planta transformation of Arabidopsis Thaliana. C. R. Acad. Sci. Paris, Life Sciences, 316, 1188 1193.
- , , , , , 1994 Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation In Planta. Plant J., 5, 551 558.
- 1996 Transformation technology. Trends Plant Sci., 1, 423 431.
- 1992 T-DNA insertion mutagenesis in Arabidopsis: Seed infection/transformation. In Methods in Arabidopsis Research ( Koncz, C. Chua, N.-H. & Schell, J., eds.). Singapore: World Scientific, pp. 274 289.
- & 1987 Agrobacterium mediated transformation of germinating seeds of Arabidopsis thaliana: a non-tissue culture approach. Mol. Gen. Genet., 208, 1 9.
- , , 1994 An essential virulence protein of Agrobacterim tumefaciens, VirB4, requires an intact mononucleotide binding domain to function in transfer of T-DNA. Mol. Gen. Genet., 245, 704 715.
- , , , 1997 Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly. Proceedings Natl Acad. Sci. USA, 94, 2122 2127.
- , , , 1998 A role for the AKT1 potassium channel in plant nutrition. Science, 280, 918 921.
- , , , 1993 New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Research, 2, 208 218.
- & 1992 Agrobacterium and plant genetic engineering. Plant Mol. Biol., 19, 15 38.
- , , , , 1994 In planta transformation of Arabidopsis Thaliana. Mol. Gen. Genet., 245, 363 370.
- , , , , , , 1989 High-frequency T-DNA-mediated gene tagging in plants. Proceedings Natl Acad. Sci. USA, 86, 8467 8471.
- & 1986 The promoter of the TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector. Mol. Gen. Genet., 204, 383 396.
- , , , , 1993 RPS2, an Arabidopsis disease resistance locus specifying recognition of Pseudomonas syringae strains expressing the avirulence gene Avrrpt2. Plant Cell, 5, 865 875.
- , , 1992 Multiple copies of virG enhance the transient transformation of celery, carrot and rice tissues by Agrobacterium Tumefaciens. Plant Mol. Biol., 20, 1071 1087.
- & 1992 The Agrobacterium tumefaciens vir gene transcriptional activator virG is transcriptionally induced by acid pH and other stress stimuli. J. Bacteriol., 174, 1189 1196.
- , , , , , 1995 Promoterless gusA expression in a large number of Arabidopsis thaliana transformants obtained by the in planta infiltration method. C. R. Acad. Sci. Paris, Life Sciences, 318, 465 474.
- , , 1997 Differences in susceptibility of Arabidopsis ecotypes to crown gall disease may result from a deficiency in T-DNA integration. Plant Cell, 9, 317 333.
- , , , , , 1982 Studies on the structure of cointegrates between octopine and nopaline Ti-plasmids and their tumor-inducing properties. Plant Mol. Biol., 1, 265 276.
- , , , , , 1998 T-DNA tagging of a flowering-time gene and improved gene transfer by in planta transformation of Arabidopsis. Aust. J. Plant Physiol., 25, 125 130.
- & 1996 Transgenic plants: genetic transformation – recent developments and state of the art. Plant Tissue Culture and Biotech., 2, 66 75.
- , , 1988 Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proceedings Natl Acad. Sci. USA, 85, 5536 5540.
- , , 1988 Foreign genes in plants: transfer, structure, expression and applications. Annu. Rev. Genet., 22, 421 477.
- , , , 1991 Identification of Pseudomonas syringae pathogens of Arabidopsis thaliana and a bacterial gene determining avirulence on both Arabidopsis and soybean. Plant Cell, 3, 49 59.
- 1992 Two-way chemical signaling in Agrobacterium–plant interactions. Microbiol. Rev., 56, 12 31.

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