Present address: Institute for Cell and Molecular Biology, Swann Building, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, UK.
Spatio-temporal analysis of mitotic activity with a labile cyclin–GUS fusion protein
Article first published online: 28 JAN 2003
The Plant Journal
Volume 20, Issue 4, pages 503–508, November 1999
How to Cite
Colón-Carmona, A., You, R., Haimovitch-Gal, T. and Doerner, P. (1999), Spatio-temporal analysis of mitotic activity with a labile cyclin–GUS fusion protein. The Plant Journal, 20: 503–508. doi: 10.1046/j.1365-313x.1999.00620.x
- Issue published online: 28 JAN 2003
- Article first published online: 28 JAN 2003
- Received 27 May 1999; revised 1 October 1999; accepted 5 October 1999.
Plant growth responds rapidly to developmental and environmental signals, but the underlying changes in cell division activity are poorly understood. A labile cyclin-GUS reporter was developed to facilitate the spatio-temporal analysis of cell division patterns. The chimeric reporter protein is turned over every cell cycle and hence its histochemical activity accurately reports individual mitotic cells. Using Arabidopsis plants transformed with cyclin-GUS, we visualized patterns of mitotic activity in wounded leaves which suggest a role for cell division in structural reinforcement.