Hyperpolarisation-activated calcium currents found only in cells from the elongation zone of Arabidopsis thaliana roots
Article first published online: 25 DEC 2001
The Plant Journal
Volume 21, Issue 2, pages 225–229, January 2000
How to Cite
Kiegle, E., Gilliham, M., Haseloff, J. and Tester, M. (2000), Hyperpolarisation-activated calcium currents found only in cells from the elongation zone of Arabidopsis thaliana roots. The Plant Journal, 21: 225–229. doi: 10.1046/j.1365-313x.2000.00659.x
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
- Received 21 October 1999; revised 26 November 1999; accepted 2 December 1999.
Calcium currents across the plasma membrane of plant cells allow transduction of environmental signals as well as nutritive calcium uptake. Using transgenic Arabidopsis plants with cell-specific expression of green fluorescent protein (GFP), we analyzed whole cell calcium currents in epidermal cells of the rapidly growing root apex, mature epidermal cells, cortical and epidermal cells from the elongation zone, and mature pericycle cells. In cells only from the rapidly growing root apex, a hyperpolarisation-activated calcium current was identified. This current was irreversibly inhibited by 10 μm Al3+, as well as being inhibited by 1 m m Co2+ and 100 μm verapamil. In no cells could a depolarisation-activated current be attributed to calcium influx. In the growing root apex, the hyperpolarisation-activated calcium current may function to allow constitutive uptake of calcium for rapid cell division and elongation.