Differential regulation of three functional ammonium transporter genes by nitrogen in root hairs and by light in leaves of tomato

Authors

  • Nicolaus Von Wirén,

    Corresponding author
    1. Pflanzenphysiologie, Zentrum für Molekularbiologie der Pflanzen Tübingen (ZMBP), Morgenstelle 1, 72076 Tübingen, Germany, and
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  • Frank-Roman Lauter,

    1. Pflanzenphysiologie, Zentrum für Molekularbiologie der Pflanzen Tübingen (ZMBP), Morgenstelle 1, 72076 Tübingen, Germany, and
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    • Present address: Bioinside GmbH, Warthestr. 21, D-14513 Berlin-Teltow, Germany.

  • Olaf Ninnemann,

    1. Pflanzenphysiologie, Zentrum für Molekularbiologie der Pflanzen Tübingen (ZMBP), Morgenstelle 1, 72076 Tübingen, Germany, and
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    • Present address: Anatomisches Institut, Charité, Humboldt Universität, D-10098 Berlin, Germany.

  • Bernd Gillissen,

    1. Pflanzenphysiologie, Zentrum für Molekularbiologie der Pflanzen Tübingen (ZMBP), Morgenstelle 1, 72076 Tübingen, Germany, and
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    • §

      Present address: Robert Rössle-Klinik–Hämatologie, Charité, Humboldt Universität, D-13125 Berlin, Germany.

  • Pia Walch-Liu,

    1. Institut für Pflanzenernährung, Universität Hohenheim, 70593 Stuttgart, Germany
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  • Christof Engels,

    1. Institut für Pflanzenernährung, Universität Hohenheim, 70593 Stuttgart, Germany
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  • Wolfgang Jost,

    1. Pflanzenphysiologie, Zentrum für Molekularbiologie der Pflanzen Tübingen (ZMBP), Morgenstelle 1, 72076 Tübingen, Germany, and
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  • Wolf B. Frommer

    1. Pflanzenphysiologie, Zentrum für Molekularbiologie der Pflanzen Tübingen (ZMBP), Morgenstelle 1, 72076 Tübingen, Germany, and
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*For correspondence (fax +49 7071 293295; e-mail vonwiren@uni-tuebingen.de).

Summary

To elucidate the role of transporters in N nutrition of tomato, two new transporter genes were isolated from cDNA libraries of root hairs or leaves of tomato. While LeAMT1;2 is closely related to LeAMT1;1 (75.6% amino acid identity), LeAMT1;3 is more distantly related (62.8% identity) and possesses two short upstream open reading frames in the 5′ end of the mRNA and a particularly short N-terminus of the protein as unique features. When expressed in yeast mutants defective in uptake, all three genes complemented uptake. In roots of hydroponically grown plants, transcript levels of LeAMT1;2 increased after or NO3 supply, while LeAMT1;1 was induced by N deficiency coinciding with low glutamine concentrations, and LeAMT1;3 was not detected. In aeroponic culture, expression of LeAMT1;1 and LeAMT1;2 was higher in root hairs than in the remaining root fraction. Growth of plants at elevated CO2 slightly decreased expression of LeAMT1;2 and LeAMT1;3 in leaves, but strongly repressed transcript levels of chloroplast glutamine synthetase and photorespiratory serine hydroxymethyl-transferase. Expression of LeAMT1;2 and LeAMT1;3 showed a reciprocal diurnal regulation with highest transcript levels of LeAMT1;3 in darkness and highest levels of LeAMT1;2 after onset of light. These results indicate that in tomato at least two high-affinity transporters, LeAMT1;1 and LeAMT1;2, are differentially regulated by N and contribute to root hair-mediated acquisition from the rhizosphere. In leaves, the reciprocally expressed transporters LeAMT1;2 and LeAMT1;3 are supposed to play different roles in N metabolism, uptake and/or NH3 retrieval during photorespiration.

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