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Differential regulation of three functional ammonium transporter genes by nitrogen in root hairs and by light in leaves of tomato
Article first published online: 25 DEC 2001
The Plant Journal
Volume 21, Issue 2, pages 167–175, January 2000
How to Cite
Von Wirén, N., Lauter, F.-R., Ninnemann, O., Gillissen, B., Walch-Liu, P., Engels, C., Jost, W. and Frommer, W. B. (2000), Differential regulation of three functional ammonium transporter genes by nitrogen in root hairs and by light in leaves of tomato. The Plant Journal, 21: 167–175. doi: 10.1046/j.1365-313x.2000.00665.x
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
- Received 28 May 1999; revised 29 November 1999; accepted 6 December 1999.
- Cited By
To elucidate the role of transporters in N nutrition of tomato, two new transporter genes were isolated from cDNA libraries of root hairs or leaves of tomato. While LeAMT1;2 is closely related to LeAMT1;1 (75.6% amino acid identity), LeAMT1;3 is more distantly related (62.8% identity) and possesses two short upstream open reading frames in the 5′ end of the mRNA and a particularly short N-terminus of the protein as unique features. When expressed in yeast mutants defective in uptake, all three genes complemented uptake. In roots of hydroponically grown plants, transcript levels of LeAMT1;2 increased after or NO3– supply, while LeAMT1;1 was induced by N deficiency coinciding with low glutamine concentrations, and LeAMT1;3 was not detected. In aeroponic culture, expression of LeAMT1;1 and LeAMT1;2 was higher in root hairs than in the remaining root fraction. Growth of plants at elevated CO2 slightly decreased expression of LeAMT1;2 and LeAMT1;3 in leaves, but strongly repressed transcript levels of chloroplast glutamine synthetase and photorespiratory serine hydroxymethyl-transferase. Expression of LeAMT1;2 and LeAMT1;3 showed a reciprocal diurnal regulation with highest transcript levels of LeAMT1;3 in darkness and highest levels of LeAMT1;2 after onset of light. These results indicate that in tomato at least two high-affinity transporters, LeAMT1;1 and LeAMT1;2, are differentially regulated by N and contribute to root hair-mediated acquisition from the rhizosphere. In leaves, the reciprocally expressed transporters LeAMT1;2 and LeAMT1;3 are supposed to play different roles in N metabolism, uptake and/or NH3 retrieval during photorespiration.