Sense transformation reveals a novel role for class I β-1,3-glucanase in tobacco seed germination
Article first published online: 25 DEC 2001
The Plant Journal
Volume 23, Issue 2, pages 215–221, July 2000
How to Cite
Leubner-Metzger, G. and Meins, F. (2000), Sense transformation reveals a novel role for class I β-1,3-glucanase in tobacco seed germination. The Plant Journal, 23: 215–221. doi: 10.1046/j.1365-313x.2000.00773.x
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
- Received 21 March 2000; accepted 5 April 2000.
- abscisic acid;
- endosperm-limited seed germination;
- gene function;
- sense transformation
‘Coat-enhanced’ seed dormancy of many dicotyledonous species, including tobacco, is released during after-ripening. Rupture of the endosperm, which is the limiting step in tobacco seed germination, is preceded by induction of class I β-1,3-glucanase (βGLU I) in the micropylar endosperm where the radicle will penetrate. Treating after-ripened tobacco seeds with abscisic acid (ABA) delays endosperm rupture and inhibits βGLU I induction. Sense transformation with a chimeric ABA-inducible βGLU I transgene resulted in over-expression of βGLU I in seeds and promoted endosperm rupture of mature seeds and of ABA-treated after-ripened seeds. Taken together, these results provide direct evidence that βGLU I contributes to endosperm rupture. Over-expression of βGLU I during germination also replaced the effects of after-ripening on endosperm rupture. This suggests that regulation of βGLU I by ABA signalling pathways might have a key role in after-ripening.