Over-expression of a single Ca2+-dependent protein kinase confers both cold and salt/drought tolerance on rice plants
Article first published online: 25 DEC 2001
The Plant Journal
Volume 23, Issue 3, pages 319–327, August 2000
How to Cite
Saijo, Y., Hata, S., Kyozuka, J., Shimamoto, K. and Izui, K. (2000), Over-expression of a single Ca2+-dependent protein kinase confers both cold and salt/drought tolerance on rice plants . The Plant Journal, 23: 319–327. doi: 10.1046/j.1365-313x.2000.00787.x
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
- Received 4 February 2000; revised 17 April 2000; accepted 17 April 2000.
- stress tolerance
A rice gene encoding a calcium-dependent protein kinase (CDPK), OsCDPK7, was induced by cold and salt stresses. To elucidate the physiological function of OsCDPK7, we generated transgenic rice plants with altered levels of the protein. The extent of tolerance to cold and salt/drought stresses of these plants correlated well with the level of OsCDPK7 expression. Therefore, OsCDPK7 was shown to be a positive regulator commonly involved in the tolerance to both stresses in rice. Over-expression of OsCDPK7 enhanced induction of some stress-responsive genes in response to salinity/drought, but not to cold. Thus, it was suggested that the downstream pathways leading to the cold and salt/drought tolerance are different from each other. It seems likely that at least two distinct pathways commonly use a single CDPK, maintaining the signalling specificity through unknown post-translational regulation mechanisms. These results demonstrate that simple manipulation of CDPK activity has great potential with regard to plant improvement.