Functional identification of an Arabidopsis Snf4 ortholog by screening for heterologous multicopy suppressors of snf4 deficiency in yeast
Article first published online: 25 DEC 2001
The Plant Journal
Volume 23, Issue 1, pages 115–122, July 2000
How to Cite
Kleinow, T., Bhalerao, R., Breuer, F., Umeda, M., Salchert, K. and Koncz, C. (2000), Functional identification of an Arabidopsis Snf4 ortholog by screening for heterologous multicopy suppressors of snf4 deficiency in yeast. The Plant Journal, 23: 115–122. doi: 10.1046/j.1365-313x.2000.00809.x
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
- Received 22 December 1999; revised 12 April 2000; accepted 18 May 2000.
- Snf1-related protein kinases;
- suppressors of snf4;
- glucose signaling;
- protein interaction;
- yeast two-hybrid system
Yeast Snf4 is a prototype of activating γ-subunits of conserved Snf1/AMPK-related protein kinases (SnRKs) controlling glucose and stress signaling in eukaryotes. The catalytic subunits of Arabidopsis SnRKs, AKIN10 and AKIN11, interact with Snf4 and suppress the snf1 and snf4 mutations in yeast. By expression of an Arabidopsis cDNA library in yeast, heterologous multicopy snf4 suppressors were isolated. In addition to AKIN10 and AKIN11, the deficiency of yeast snf4 mutant to grown on non-fermentable carbon source was suppressed by Arabidopsis Myb30, CAAT-binding factor Hap3b, casein kinase I, zinc-finger factors AZF2 and ZAT10, as well as orthologs of hexose/UDP-hexose transporters, calmodulin, SMC1-cohesin and Snf4. Here we describe the characterization of AtSNF4, a functional Arabidopsis Snf4 ortholog, that interacts with yeast Snf1 and specifically binds to the C-terminal regulatory domain of Arabidopsis SnRKs AKIN10 and AKIN11.