Expression of Clarkia S-linalool synthase in transgenic petunia plants results in the accumulation of S-linalyl-β-d-glucopyranoside
Article first published online: 23 DEC 2001
The Plant Journal
Volume 27, Issue 4, pages 315–324, 2001
How to Cite
Lücker, J., Bouwmeester, H. J., Schwab, W., Blaas, J., Van Der Plas, L. H. W. and Verhoeven, H. A. (2001), Expression of Clarkia S-linalool synthase in transgenic petunia plants results in the accumulation of S-linalyl-β-d-glucopyranoside. The Plant Journal, 27: 315–324. doi: 10.1046/j.1365-313x.2001.01097.x
- Issue published online: 23 DEC 2001
- Article first published online: 23 DEC 2001
- Received 14 March 2001; revised 18 May 2001; accepted 22 May 2001.
- Petunia hybrida;
- pathway engineering;
- liquid chromatography-tandem MS;
- S-linalool synthase.
Petunia hybrida W115 was transformed with a Clarkia breweri S-linalool synthase cDNA (lis). Lis was expressed in all tissues analysed, and linalool was detected in leaves, sepals, corolla, stem and ovary, but not in nectaries, roots, pollen and style. However, the S-linalool produced by the plant in the various tissues is not present as free linalool, but was efficiently converted to non-volatile S-linalyl-β-d-glucopyranoside by the action of endogenous glucosyltransferase. The results presented demonstrate that monoterpene production can be altered by genetic modification, and that the compounds produced can be converted by endogenous enzymatic activity.