Immunoperoxidase technique for detecting spirochetes in tissue sections: comparison with other methods
Article first published online: 25 DEC 2001
International Journal of Dermatology
Volume 39, Issue 8, pages 609–613, August 2000
How to Cite
Phelps, R. G., Knispel, J., Tu, E. S., Cernainu, G. and Saruk, M. (2000), Immunoperoxidase technique for detecting spirochetes in tissue sections: comparison with other methods. International Journal of Dermatology, 39: 609–613. doi: 10.1046/j.1365-4362.2000.00029.x
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
Background With the increasing incidence of human immunodeficiency virus (HIV) infection and immunosuppressive therapy, the incidence of syphilis has been increasing. Given the fact that the above conditions may mask or obscure the usual clinical signs and symptoms of syphilis, a means of enhanced detection is essential.
Aims methods The purpose of this study was to determine whether an immunoperoxidase method using an antibody against treponemes would increase the sensitivity and specificity of diagnosis in biopsies of patients with secondary syphilis. This was compared to serology and silver stain in cases of known syphilis.
Results Immunoperoxidase for treponemes was at least as sensitive (9/10) as pathology (9/10), and more sensitive than conventional silver stain (6/10) or serology (7/10).
Conclusions In those equivocal cases of secondary syphilis, where confirmation is essential, immunoperoxidase for treponemes may be a useful adjunct.