TNFα and IL-6 are mediators in the blistering process of pemphigus

Authors

  • Eréndira López-Robles mSc,

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      *These authors contributed equally
  • Esperanza Avalos-Díaz md ,

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      *These authors contributed equally
  • Elisa Vega-Memije md ,

    1. From the Department of Immunology, CBE, Universidad Autónoma de Zacatecas, Guadalupe, Zac. México, and the Department of Dermatology, Hospital General'Dr Manuel Gea González,' SS. México
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  • Teresa Hojyo-Tomoka md ,

    1. From the Department of Immunology, CBE, Universidad Autónoma de Zacatecas, Guadalupe, Zac. México, and the Department of Dermatology, Hospital General'Dr Manuel Gea González,' SS. México
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  • Ricardo Villalobos bsc ,

    1. From the Department of Immunology, CBE, Universidad Autónoma de Zacatecas, Guadalupe, Zac. México, and the Department of Dermatology, Hospital General'Dr Manuel Gea González,' SS. México
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  • Saúl Fraire msc ,

    1. From the Department of Immunology, CBE, Universidad Autónoma de Zacatecas, Guadalupe, Zac. México, and the Department of Dermatology, Hospital General'Dr Manuel Gea González,' SS. México
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  • Luciano Domíguez-Soto md ,

    1. From the Department of Immunology, CBE, Universidad Autónoma de Zacatecas, Guadalupe, Zac. México, and the Department of Dermatology, Hospital General'Dr Manuel Gea González,' SS. México
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  • Rafael Herrera-Esparza md.

    1. From the Department of Immunology, CBE, Universidad Autónoma de Zacatecas, Guadalupe, Zac. México, and the Department of Dermatology, Hospital General'Dr Manuel Gea González,' SS. México
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Esperanza Avalos-Díaz, MD
Chepinque 306
Col. Lomas de la Soledad
Zacatecas
Zac. 98040
México
E-mail: avalose@cantera.reduaz.mx
*These authors contributed equally

Abstract

Background  Pemphigus is an autoimmune disease characterized by intraepidermal blisters induced by pemphigus IgG. In addition to autoantibodies, molecular mechanisms involved in acantholysis remain largely unknown. For this reason, we address a possible role of the inflammatory cytokines IL-6 and TNFα in pemphigus lesions.

Methods  Sixteen biopsies from patients with different types of pemphigus were studied by in situ hybridization using DNA fluorescent probes for IL-6 and TNFα mRNA.

Results  Fifty-six percent of lesional biopsies exhibited cytokine gene expression, which was poorly expressed in noninvolved skin. Deposits of TNFα and IL-6 were products of in situ transcription at the epidermal level.

Conclusions  Inflammatory cytokine expression around the blister could play a mediator role in pemphigus lesions by increasing epithelial damage.

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