T. E. Reimchen, Department of Biology, University of Victoria, PO Box 3020, Victoria, British Columbia, Canada, V8W 3N5. Tel.: +1 250 721 7101; e-mail: firstname.lastname@example.org
Individuals with random left–right departures from bilateral symmetry are predicted to exhibit fitness reduction including increased parasitism. In an insular lake population of stickleback (Gasterosteus aculeatus) from the Queen Charlotte Islands, Western Canada, phenotypes with high or low number of lateral bony plates exhibited increased plate asymmetry relative to modal phenotypes. Asymmetric lateral plate phenotypes had increased prevalence and to a lesser extent intensity of parasitism relative to symmetric individuals, suggesting that differences in genetic resistance to pathogens contributed to unequal parasitism. The effect occurred mainly in the larger adults and during the warmest season, which may be due to the high metabolic costs incurred during the summer breeding season. Dietary differences between symmetric and asymmetric phenotypes were also detected and could contribute to unequal infection rates by mediating exposure to infected prey items. Our study, which is one of the first long-term field assessments of asymmetry and parasitism, yields results that are consistent with studies linking asymmetry to reduced fitness and indicate that lateral plate asymmetry can be an indicator of poor individual quality, despite its apparent directionality.
Threespine stickleback (Gasterosteus aculeatus) have been used as a model system in a diversity of evolutionary and ecological studies (Wootton, 1984; Bell & Foster, 1994). One of the well-studied traits on these fish are the bony lateral plates. These serially arranged plates, which usually extend over the anterior trunk, are part of the antipredator defence apparatus and can vary in number among individuals and among populations according to local selective regimes (Hagen & Gilbertson, 1972; Moodie & Reimchen, 1976; Gross, 1978; Reimchen, 1983, 1994; for review). Bilateral asymmetry in number of plates also occurs among individuals and while this asymmetry appears to be heritable (Hagen, 1973), the frequency distribution of asymmetries is usually normally distributed and approximates ideal FA (Moodie & Reimchen, 1976). General FA theory predicts that asymmetric stickleback should exhibit reduced individual quality. Evidence for this is limited and contradictory. There is a lower incidence of asymmetries in populations exposed to extensive predation by trout, suggesting fitness reduction (Moodie & Reimchen, 1976; Bergstrom & Reimchen, 2000). Alternatively, asymmetric male stickleback appear to have increased success in nesting behaviour and care of young relative to symmetric males and this leads to higher fitness (Moodie & Moodie, 1996).
As part of a long-term evolutionary investigation of endemic stickleback populations from the Queen Charlotte Islands, off the west coast of Canada, a population was monitored over 15 years for several morphological and life history parameters, including spine morphology, parasites and dietary composition (Reimchen, 1980, 1982, 1997). Stickleback were also scored for numbers of lateral plates on both sides of the body and these data provide an opportunity to test several hypotheses on asymmetry: (1) extreme lateral plate phenotypes will exhibit increased asymmetry relative to modal phenotypes; (2) asymmetrical fish will exhibit increased parasitism relative to symmetrical fish and (3) differential parasitism is influenced by dietary variation.
Materials and methods
A total of 10 204 adult (≥ 45 mm standard length – SL) stickleback collected from 1970 to 1987 were scored for sex, number of left and right lateral plates and number of dorsal, pelvic and anal spines, and number of cestodes (Schistocephalussolidus, Cyathocephalustruncatus), nematodes (Eustrongylides spp.) and trematodes (Bunodera sp., years 1981–1987 only) (details in Reimchen, 1980, 1982, 1997). Stickleback in this population are all of the low-plated morph.
Absolute lateral plate asymmetry was calculated as |R–L|. To determine whether extreme phenotypes exhibit higher levels of absolute asymmetry than modal phenotypes, we used methods outlined in Soulé & Cuzin-Roudy (1982). Results were the same whether left or right lateral plate number was used as a measure of trait size and thus we report results from analyses using the number of left lateral plates only. The distributions of both trait size and absolute asymmetry for each sex within each year were standardized (z scores, Sokal & Rohlf, 1981). Trait size values within 1 standard deviation (SD) of the mean were designated modal phenotypes, while trait values greater than 1 SD above or below the mean were designated extreme phenotypes. ANOVA was used to test for variability in asymmetry levels between the extreme and modal phenotypes. Sex and year were included as factors in the ANOVA to test whether the effect was similar between genders and among years. Results were congruent when nonparametric analyses were performed comparing the presence, rather than the level, of asymmetry between the extreme and modal phenotypes (data not shown).
We examined the statistical characteristics of signed lateral plate asymmetry (R–L) (Palmer & Strobeck, 1986; Palmer, 1994). We tested for normality using one-sample Kolmogorov-Smirnov tests (K–S), for directional asymmetry (DA) using one sample t-tests, binomial tests and measures of skewness (g1, Sokal & Rohlf, 1981) and for antisymmetry using measures of kurtosis (g2). Absolute lateral plate asymmetry was used in all subsequent analyses.
Stickleback from Boulton Lake exhibit size- and gender-related variability in the number of dorsal (1–3) and pelvic (0–2) spines and in levels of parasitism (Reimchen, 1980, 1997). However, the presence or absence of plate asymmetry was independent of spine morph and size class in males (morph, χ28=8.87, P=0.35, n=6059; size class, χ22=1.95, P=0.38, n= 6059) and in females (morph, χ28=13.29, P=0.10, n=4145; size class, χ22=0.03, P=0.99, n=4145).
We compared prevalence (% infected) and intensity (numbers of parasites per individual) of parasitic infection between symmetric and asymmetric stickleback. Patterns of parasitism in Boulton Lake vary seasonally (Reimchen, 1982) and, consequently, we performed all analyses involving parasite prevalence or intensity in four seasons (Winter, Jan.–Mar.; Spring, Apr.–Jun.; Summer, Jul.–Sept.; Fall, Oct.–Dec.). For the three parasite species scored throughout the entire sample period (S. solidus, C. truncatus, nematodes), we analysed individual and total parasitism (all spp.). For the single species analyses with S. solidus, C. truncatus and nematodes we exclude fish that were also infected with one or both of the other two species. For analyses with Bunodera sp., which was only scored systematically from years 1981–1987, we exclude all multiple species infections.
We tested for associations between lateral plate asymmetry (yes–no) and the prevalence of parasitism using log-likelihood G-tests (Sokal & Rohlf, 1981) and loglinear multiway contingency analyses (LL). The loglinear model included five factors: lateral plate asymmetry (symmetric/asymmetric), parasite status (uninfected/infected), gender (male/female), size (<57 mm SL,=57 mm SL) and sample year. To test for associations between asymmetry and parasite intensity we excluded uninfected stickleback and assigned infected fish from each species into one of two infection categories (1 parasite; > 1 parasite). Interactions were tested with G-tests and loglinear analyses, replacing parasite status with parasite intensity. To test whether the effect of an interaction in the loglinear analysis was zero we used partial chi-square values (Norusis, 1993). We present two-way interactions between asymmetry and parasitism as well as all significant higher-order interactions involving asymmetry and parasitism. The directionality of trends among years (% asymmetrical infected, % symmetrical infected) was tested using Wilcoxon’s Signed Rank tests (WR).
We also compared the diet of symmetric and asymmetric lateral plate phenotypes. The stomach contents of uninfected adult stickleback (n=3753), previously scored for the presence or absence of various food items (Reimchen, 1982), were grouped into two major categories: (1) zooplankton (cladocerans, copepods); (2) macrobenthos (chironomids, amphipods, zygopterans and trichopterans). We used G-tests to compare the proportion of these food types between symmetric and asymmetric stickleback overall and partitioned for gender and season. As pelagic copepods (Diaptomus spp.) and benthic amphipods (Gammerus spp.) act as primary hosts for S. solidus and C. truncatus, respectively (Clarke, 1954; Vik, 1958), we also compared the consumption of these specific food items. The primary hosts of nematodes are benthic oligochaetes (Hoffman, 1967) and hosts for Bunodera are variable, including molluscs and ostracods (Cannon, 1971; Skorping, 1981), but these items were not detected in stickleback stomachs. All statistical analyses were done using SPSS (v.9.0) and all significance levels are two-tailed.
Lateral plate number and general character asymmetry
Lateral plate number varied from 0 to 6 among individuals with a mode at four plates (Fig. 1A). Stickleback with low or high plate number exhibited much higher levels of asymmetry than modal phenotypes (Fig. 1B, F6,10198=149.72, P < 0.001) and this occurred in all 15 years of sampling (P < 0.001; Table 1). Differences between the extreme and modal phenotypes were accentuated in males (0.59, –0.12, respectively) relative to females (0.47, –0.12, respectively).
Table 1. Results of three-factor ANOVA testing for significant variability in standardized absolute lateral plate asymmetry between extreme and modal lateral plate phenotypes in Boulton Lake stickleback (classification of phenotypes according to procedures outlined in Soulé & Cuzin-Roudy, 1982; see Methods). There was significant variability in lateral plate asymmetry between extreme and modal phenotypes, with extreme phenotypes exhibiting higher levels of asymmetry (see Fig. 1B). This was accentuated in males but was not dependent on sample year.
The frequency distribution of right and left asymmetry was approximately normal (Fig. 2; Table 2) but signed (R–L) asymmetry exhibited a slight left-side bias in males and females (0.55, 0.57 left side departures, respectively, both P < 0.001, Binomial tests). Signed lateral plate asymmetry was significantly different from zero (both sexes, t10203=–9.04, P < 0.001; males, t6058=–5.70, P < 0.001; females, t4144=–7.19, P < 0.001, one-sample t-test). This left-side bias was greater in females than in males (t10202=2.28, P < 0.05), as was absolute asymmetry (Z=–4.78, P < 0.001, Mann–Whitney U-test; Fig. 3). The extent of the directionality and of the differences between the genders fluctuated among years (Fig. 3), with lateral plate asymmetry in some years often approximating ideal FA (i.e. mean signed asymmetry=0). We detected no significant skewness when the sexes were combined (t∞=1.17, P > 0.20) or when males and females were treated separately (males, t∞=1.32, P > 0.10; females, t∞=0.05, P > 0.80). Although statistical departures from normality were detected (overall, Z=24.35, P < 0.001; males, Z=19.36, P < 0.001; females, Z=14.75, P < 0.001, K–S), the distribution of unsigned asymmetry was leptokurtic (overall, t∞=12.77, P < 0.001; males, t∞=11.16, P < 0.001; females, t∞=6.32, P < 0.001), indicating no evidence of antisymmetry.
Table 2. Descriptive statistics for the number of right (rlp) and left (llp) lateral plates and for signed (R-L) and absolute |R−L| asymmetry in the Boulton Lake stickleback. Values in parentheses are SD except for skew and kurtosis where values in parentheses are SE. Results are given for the population as a whole (n = 10 204) and for each gender separately (males, n = 6059; females, n = 4145). See text for statistical tests concerning asymmetry and Fig. 2 for yearly means.
We detected increased parasitism in asymmetric stickleback but such associations were dependent on season and size class. Symmetric and asymmetric stickleback exhibited similar infections in each of the four seasons and we detected no two-way interactions between lateral plate symmetry and parasite status (all partial χ21 < 2.00, all P > 0.05). However, there was a three-way interaction between parasitism, asymmetry and size class during summer (partial χ21=3.91, n=3263, P < 0.05, LL; Fig. 4). In the smaller fish (<57 mm), symmetric and asymmetric stickleback exhibited similar infection in both sexes (males, 21.1%, 20.7%, respectively, G=0.04, d.f.=1, P=0.83, n=1743; females, 28.0%, 28.2%, respectively, G=0.00, d.f.=1, P=0.95, n=1100, G-tests). In the larger fish (≥57 mm), asymmetric stickleback exhibited increased parasite prevalence relative to symmetric individuals (63.6%, 52.5%, respectively, G=5.35, d.f.=1, P < 0.05, n=420). This differential was accentuated in males (58.4%, 44.0%, respectively, G=5.61, d.f.=1, P < 0.05, n=271) compared with females (72.3%, 69.7%, respectively, G=0.12, d.f.=1, P=0.73, n=149, G-tests).
Individuals with high or low lateral plate number exhibited increased asymmetry and, consequently, we tested whether overall parasite prevalence or the increased parasitism of large asymmetric fish (all spp.) during summer was influenced by lateral plate number. A loglinear analysis with lateral plate number (1–2, 3–4, 5–6) as a factor revealed parasite prevalence was independent of lateral plate number (all interactions with plate number, P > 0.15). In fact, the three-way interaction between parasitism, asymmetry and size class was improved (partial χ21=5.07, P < 0.05, n=3263, LL).
Trends in single species parasites comparisons were congruent with those from total parasitism. Among pooled size classes, no significant interactions between lateral plate symmetry and parasitic infection were detected in any season (all partial χ2 < 3.00, all P > 0.05). When partitioned for size, asymmetric stickleback exhibited increased parasite prevalence relative to symmetric fish in large but not in small fish (Table 3). None of the three–way interactions between lateral plate condition, parasite status and size class during summer reach statistical significance (S. solidus, partial χ21=2.04, P=0.15, n=3016; C. truncatus, partial χ21=3.28, P= 0.07, n=2370; nematodes, partial χ21=1.65, P=0.20, n=2467; Bunodera sp., partial χ21=0.35, P=0.55, n=684) but the differential occurred for all four species of parasite (combined probabilities, χ28=15.70, P < 0.05, Sokal & Rohlf, 1981). Partitioning these data for gender suggests an interaction, as pronounced differences in infection in the large fish occurred for all four parasite species in males but only for nematodes and Bunodera sp. in females (Fig. 4). However, a higher order statistical interaction with sex was not detected for any of the parasite species in the loglinear analyses (all P > 0.05).
Table 3. Parasite prevalence for symmetric (S) and asymmetric (AS) stickleback collected during the summer (Jul.–Sept.) season. Infection frequencies tend towards equality in the smaller (<57 mm) fish but are reduced in symmetric vs. asymmetric stickleback for the larger fish (≥57 mm, combined probabilities, P < 0.05). G-values shown are from log-likelihood G-tests for each parasite species and size class.
The differential parasitism observed in large fish during summer showed temporal consistency as asymmetric fish had increased parasite prevalence in 8 of 10 years (Z=–1.785, P=0.07, WR; heterogeneity among years – partial χ215=5.83, P=0.98, n=3263; Fig. 5). Among smaller fish, trends were less consistent among years (Z=–0.94, P=0.35, WR).
Among infected stickleback (n=3270), asymmetric individuals exhibited increased parasite intensity, but this was dependent on species of parasite, season and sex. For S. solidus, we did not detect any overall association between lateral plate asymmetry and parasite intensity in any of the four seasons (all partial χ2 < 1.55, all P > 0.05). However, in summer increased parasitism of asymmetric stickleback was dependent on sex (partial χ21=7.44, P < 0.05, n=672) and on year (partial χ215=27.10, P < 0.05, n=672). There was no statistical effect in males (G=0.88, d.f.=1, P=0.35, n=386) but, in females, asymmetric fish exhibited increased frequencies of multiple parasite infection relative to symmetric individuals (57.3%, 44.2%, respectively, G=4.91, d.f.=1, P < 0.05, n=286) and this occurred in 7 of 10 years.
For C. truncatus, we detected an association between lateral plate asymmetry and parasite intensity in winter (partial χ21=9.47, P < 0.01, n=362), but not in the other three seasons (all partial χ2 < 2.00, all P > 0.10). Unexpectedly, symmetric fish had higher levels of multiple parasite infections than asymmetric fish (50.6%, 37.5%, respectively). There were few infected females during winter and these exhibited similar infection intensities between symmetric and asymmetric individuals (25.0%, 25.8%, respectively, G=0.01, d.f.=1, P=0.94, n=63, G-test). Thus the interaction detected in the loglinear analysis was essentially due to increased levels of multiple parasite infections in symmetric vs. asymmetric male stickleback. This occurred in both size classes (< 57 mm, 51.1%, 38.6%, respectively, G=3.39, d.f.=1, P=0.07, n=219; ≥ 57 mm, 71.1%, 42.9%, respectively, G=6.56, d.f.=1, P < 0.01, n=80, G-tests) and was consistent across years (lateral plate asymmetry × parasite intensity–year interaction, partial χ27=8.25, d.f.=1, P=0.41).
Asymmetrical females exhibited increased multiple nematode infections relative to symmetric females in the seasons where these parasites were consistently detected in (Fig. 6). Partitioning the data by gender and pooling the results across seasons revealed a significant excess of multiple parasite infections in asymmetric vs. symmetric females (51.4%, 32.8%, respectively, G=4.56, d.f.=1, P < 0.05, n=128, G-test) and no significant differences in males (36.2%, 44.3%, G=0.87, d.f.=1, P=0.35, n=130, G-test).
We detected increased Bunodera infection among asymmetric fish during winter (80.4%, 69.7%, respectively, partial χ21=3.88, P < 0.05, n=300) but not during the remaining seasons (partial χ2 < 2.80, P > 0.05). The effect was most expressed in females (Fig. 6). We partitioned the data by gender, pooled the results across seasons and introduced season in a five-factor loglinear analysis. In females, this revealed increased parasite intensity in asymmetric vs. symmetric individuals independent of other interactions (74.2%, 60.7%, n=336, partial χ21=6.77, P < 0.01, n=336). Yearly trends were fairly consistent as asymmetric fish exhibited increased levels of multiple parasite infection in 6 of 7 years (Z=–2.03, P < 0.05, WR). In males, we did not detect any differences in multiple infections between asymmetric and symmetric fish (62.9%, 64.3%, respectively, partial χ21=0.31, P=0.72, n=530).
Diet of uninfected stickleback
Among pooled samples, asymmetric stickleback exhibited increased consumption of macrobenthos relative to that of symmetric phenotypes (63.6%, 58.3%, G=6.23, d.f.=1, P < 0.05, n=3753) but there was similar consumption of zooplankton, copepods and amphipods (all P > 0.15). Partitioning the data shows that the amount of association was dependent on season and gender. Although macrobenthos was more common in asymmetric fish, this effect was greatest in males during summer (62.2%, 54.0%, respectively, G=4.19, d.f.=1, P < 0.05, n=601) and in females during winter (84.9%, 71.2%, respectively, G=3.94, d.f.=1, P < 0.05, n=145). During summer, zooplankton was more common in asymmetric males (57.5%, 49.3%, respectively, G=4.05, d.f.=1, P < 0.05, n=601) but, in females, it was less common (32.5%, 45.0%, respectively, G=4.42, d.f.=1, P < 0.05, n=277). Amphipods were less common in asymmetric females than symmetric females during summer (4.2%, 11.7%, respectively, G=5.45, d.f.=1, P < 0.05, n=277). All other seasonal comparisons of diet between symmetric and asymmetric fish were nonsignificant (all P > 0.05, G-tests). Including size and year in multiway contingency analyses did not reveal higher order interactions with either factor for any seasonal dietary comparison (all P > 0.05).
Four mechanisms, which are not mutually exclusive, could account for the increased parasitism of asymmetric individuals. Firstly, differential infection by parasites or disease could reflect genetic variability in immunocompetence (Wakelin, 1978; Sage et al., 1986; Møller, 1999). Such genetic differences between phenotypes may be particularly feasible for plate asymmetry, which is heritable in Gasterosteus (Hagen, 1973). Associations between plate asymmetry and plate number also support this hypothesis. As reduction in lateral plate number or symmetry may have functional implications (i.e. reduced defence against predators, Bergstrom & Reimchen, 2000; Reimchen, 1983, 2000), we predicted associations between asymmetry and trait size (number of plates) would be indicative of traits under stabilizing selection, with extreme phenotypes exhibiting increased asymmetry (Møller, 1991; Balmford et al., 1993; Møller & Pomiankowski, 1993; Crespi & Vanderkist, 1997; Clarke, 1998b). Consistent with theory and prediction, stickleback with relatively low or high lateral plate number had higher levels of asymmetry than modal phenotypes, as has been documented in other species of fish and insects (Soulé & Cuzin-Roudy, 1982; Kodric-Brown, 1997; but see Woods et al., 1999). Homozygosity has been linked to inferior developmental stability (Leary et al., 1984; Mitton, 1993; for review; but see Clarke, 1993) and Soulé (1982) suggested homozygotes could be partitioned into the tails of the size distribution for some morphological characters. Immunological defences against infection are accentuated by enzyme heterozygosity (Tooby, 1982; Thornhill & Gangestad, 1993; Mitton, 1995). If stickleback at the edge of the frequency distribution for lateral plates, which exhibit high levels of asymmetry, are highly homozygous relative to modal phenotypes, they may have reduced resistance to parasites and such associations provide one plausible mechanism to account for the increased parasitism of asymmetric stickleback observed in our study.
However, alternative explanations for the associations between trait size and asymmetry exist. Genotypes with extreme phenotypes may contain poorly balanced or poorly integrated genes (Thoday, 1958) and exhibit weaker developmental stability as a result of such genetic imbalance (Clarke et al., 1992; Clarke, 1993; for review). Also, extreme phenotypes may exhibit higher FA if individuals with extremely large or small morphological structures suffer greater stress during development as a result of bearing traits of suboptimal size (Møller & Pomiankowski, 1993). If asymmetrical structures result in reduced performance (e.g. Møller, 1991; Balmford et al., 1993), this may cause even greater stress during subsequent development. Conceivably, elevated FA in extreme phenotypes results from a combination of these factors, each of which may influence relative parasitism of asymmetric phenotypes.
Secondly, increased infection of highly asymmetric phenotypes could simply reflect increased exposure to infectious agents (Bonn et al., 1996). Consistent with prediction, we detected some dietary differences between symmetric and asymmetric phenotypes that could influence encounter rates with infected prey items. For example, zooplankton consumption in summer was biased towards asymmetric plate phenotypes in large males but towards symmetric phenotypes in large females. Only asymmetric males exhibited increased prevalence of S. solidus and this could occur if the relatively more pelagic diet of asymmetric males, but not asymmetric females, results in high encounter rates between asymmetric males and the pelagic copepod that acts as primary host for S. solidus. Also, asymmetric adult males, but not asymmetric females, had increased C. truncatus infections relative to symmetric fish during summer. Reduced consumption of amphipods, the primary host of C. truncatus, by asymmetric females relative to symmetric females could contribute to the lack of excess parasite prevalence observed in asymmetric females.
Such associations between diet and parasitism are consistent with results for symmetric and asymmetric pelvic spine phenotypes in subadult stickleback and for differential parasitism between the sexes, where dietary variation appears to contribute to differential parasitism (Reimchen, 1997; Reimchen & Nosil, 2001a, b). Competition for resources is one mechanism that could lead to ecological differences among individuals differing in levels of asymmetry (e.g. Møller, 1995; Nosil & Reimchen, 2001). Our results suggest ecological sources of differential parasitism or fitness among individuals varying in levels of asymmetry may be of greater importance than currently appreciated. If so, this may account for the increased C. truncatus intensity in symmetric vs. asymmetric males, a trend opposite to that predicted by theory (see also Reimchen, 1997).
Thirdly, asymmetry may itself be a signal of increased environmental stress during development and/or poor phenotypic condition, and both these factors can result in decreased immunocompetence (Gershwin et al., 1985; Parsons, 1990; for review; Herbert & Cohen, 1993; Badyaev et al., 2000; Nosil & Reimchen, 2001). For example, we detected increased parasite prevalence in asymmetric stickleback only during summer. Metabolic costs for ectotherms are accentuated during warm weather (Wootton, 1984) and stickleback in poor phenotypic condition may be unable to mount an efficient immune response. A similar process could account for why increased parasite prevalence in asymmetric stickleback was restricted to large adult fish, which bear the costs of reproduction (Wootton & Evans, 1976; Fitzgerald et al., 1989; Bakker, 1994). Costs of reproduction may also explain why differences were more pronounced in males than females, as male stickleback provide all the parental care and exhibit all the territorial defence (De-Fraipont et al., 1992; Bakker, 1994).
Fourthly, the costs of parasitism could result in increased physiological stress during development and thus directly induce high levels of asymmetry (Møller, 1992; Polak, 1993; Folstad et al., 1996; Markusson & Folstad, 1997). This could occur in stickleback as initial S. solidus infections can occur before and during the expression of the lateral plates. However, the general importance of this is unknown as two species of parasites (C. truncatus and Eustrongylides) occur predominantly on fish larger than 40 mm SL that have completed plate development.
In summary, stickleback with lateral plate numbers that departed most from the population mean had increased asymmetry and these asymmetrical phenotypes tended to exhibit higher prevalence and to a lesser extent higher intensity of parasitism than symmetric phenotypes. Such associations between asymmetry, trait size and parasitism suggest differences in genetic resistance to parasitic infection, possibly mediated by enzyme heterozygosity. Our results represent one of the few long-term studies of the fitness consequences of departures from symmetry and have allowed the detection of slight seasonal and age-related trends in differential parasitism between symmetric and asymmetric phenotypes. Furthermore, ecological factors influencing parasitism are rarely addressed in studies of bilateral asymmetry and our data suggest that niche and dietary differences among individuals varying in levels of asymmetry can contribute to unequal infection rates. The findings have additional implications to the expanding body of literature on asymmetry as they demonstrate that fitness reduction is not restricted to FA but can occur with traits that exhibit slight directionality.
T.E.R. thanks Natural Sciences and Engineering Research Council (NSERC) of Canada (A2354) for support. P.N. also thanks NSERC (PGS A) for support.