24-hour 51Cr post-transfusion survival, 51Cr life span and haemolysis of red blood cells stored at 4 °C for 56 days in AS-3

Authors

  • C. R. Valeri,

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    • The opinions or assertions contained herein are those of the authors and are not to be construed as official or reflecting the views of the Navy Department or Naval Service at large.

  • L. E. Pivacek,

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    • The opinions or assertions contained herein are those of the authors and are not to be construed as official or reflecting the views of the Navy Department or Naval Service at large.

  • G. P. Cassidy,

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    • The opinions or assertions contained herein are those of the authors and are not to be construed as official or reflecting the views of the Navy Department or Naval Service at large.

  • G. Ragno

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    • The opinions or assertions contained herein are those of the authors and are not to be construed as official or reflecting the views of the Navy Department or Naval Service at large.


: C. Robert Valeri, Naval Blood Research Laboratory, Boston University School of Medicine, 615 Albany Street, Boston, MA 02118, USA Tel.: 617-638-4950 Fax: 617-638-4210 E-mail: navblood@BU. edu

Abstract

Background and Objectives

Red blood cells (RBC) were collected either by a manual method using a 16-gauge needle or by an apheresis procedure using an 18-gauge needle, and were stored at 4 °C in a solution of CP2D (anticoagulant)/AS-3 (Nutricel) for 56 days. The purpose was to compare the outcome of the autotransfused red cells collected by both techniques.

Materials and Methods

Five healthy male volunteers were studied on two occasions.

Results

The autotransfusions of the manual and apheresed RBC resulted in a mean 24-h post-transfusion survival of 71%, a normal mean 51Cr RBC life span, a 2,3 DPG level that was less than 10% of normal, and 0·6% haemolysis.

Conclusions

Whether collected manually or by apheresis, the outcomes were similar for RBC stored at 4 °C for 56 days in CP2D/AS-3.

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