A novel true volumetric method for the determination of residual leucocytes in blood components
Article first published online: 30 MAY 2002
Volume 82, Issue 4, pages 198–206, May 2002
How to Cite
Cassens, U., Greve, B., Tapernon, K., Nave, B., Severin, E., Sibrowski, W. and Göhde, W. (2002), A novel true volumetric method for the determination of residual leucocytes in blood components. Vox Sanguinis, 82: 198–206. doi: 10.1046/j.1423-0410.2002.00176.x
- Issue published online: 30 MAY 2002
- Article first published online: 30 MAY 2002
- Received: 6 September 2001, revised 28 January 2002, accepted 20 February 2002
- blood components;
- flow cytometer;
- microvolume fluorimetry
Background and Objectives Accurate determination of residual leucocytes [white blood cells (WBC)] in blood components is of high clinical importance. To date, several labour-intensive, time-consuming or expensive techniques have been used for this purpose.
Materials and Methods A method for the determination of residual WBC is described using a novel low-cost flow-cytometric cell counter and analyser (CCA). The DNA in WBC was stained using 4′-6-diamidino-2-phenylindole (DAPI) and WBC were automatically analysed by true volumetric counting of 200-µl samples (prepared from a 20-µl undiluted sample).
Results Dilution experiments over a range of 0·5–50 WBC/µl showed a linearity of r= 0·998. The detection limit of this method was 0·83 WBC/µl of red blood cell concentrate (RCC) and 0·67 WBC/µl of platelet concentrate (PC), with an accuracy of 95·5%.
Conclusion Residual WBC (< 1 WBC/µl) can be accurately counted using the CCA within 2 min and at a total cost of less than € 1 per sample.