Biochemical characterization of the vanilloid receptor 1 expressed in a dorsal root ganglia derived cell line
Article first published online: 19 AUG 2003
European Journal of Biochemistry
Volume 268, Issue 21, pages 5489–5496, November 2001
How to Cite
Jahnel, R., Dreger, M., Gillen, C., Bender, O., Kurreck, J. and Hucho, F. (2001), Biochemical characterization of the vanilloid receptor 1 expressed in a dorsal root ganglia derived cell line. European Journal of Biochemistry, 268: 5489–5496. doi: 10.1046/j.1432-1033.2001.02500.x
- Issue published online: 19 AUG 2003
- Article first published online: 19 AUG 2003
- (Received 31 July 2001, accepted 6 September 2001)
- vanilloid receptor 1;
- quaternary structure;
The vanilloid receptor VR1 is an ion channel predominantly expressed by primary sensory neurons involved in nociception. Here we describe its biochemical properties and assess the subcellular localization, the glycosylation state and the quaternary structure of VR1 expressed in HEK293 cells and in the DRG-derived cell line F-11 (N18TG2 mouse neuroblastoma × rat dorsal root ganglia, hybridoma). VR1 was found to be glycosylated in both cell types. Of the five potential N-glycosylation sites, the predicted transient receptor potential channel-like transmembrane folding proposes N604 is localized extracellularly. We used site-directed mutagenesis to mutate the Asn at position 604 to Thr. This mutated VR1 was not glycosylated, confirming the extracellular location of N604 and its role as the exclusive site of glycosylation of the VR1 protein. VR1 occured in high molecular mass complexes as assessed by blue native PAGE. In the presence of limited amounts of SDS dimers, trimers and tetramers of VR1 were observed, consistent with the predicted tetrameric quaternary structure of the receptor. Cross-linking with dimethyladipimidate yielded almost exclusively dimers.
Whereas VR1 localized both to the plasma membrane and to intracellular membranes in HEK293 cells, it localized predominantly to the plasma membrane in F-11 cells. Using confocal laserscanning microscopy, we observed an enrichment of anti-VR1 immunoreactivity in neurite-like structures of F-11 cells. In the light of conflicting literature data on biochemical characteristics of VR1, our data suggest that dorsal root ganglion-derived F-11 cells provide a powerful experimental system for the study of VR1 biochemistry.