• 1
    Pelosi, P. (1996) Perireceptor events in olfaction. J. Neurobiol. 30, 319.
  • 2
    Steinbrecht, R.A. (1998) Odorant-binding proteins: expression and function. Ann. N. Y. Acad. Sci. 855, 323332.
  • 3
    Krieger, J. & Breer, H. (1999) Olfactory reception in invertebrates. Science 28, 720723.
  • 4
    Campanacci, V., Krieger, J., Bette, S., Sturgis, J.N., Lartigue, A., Cambillau, C., Breer, H. & Tegoni, M. (2001) Revisiting the specificity of Mamestra brassicae and Antheraea polyphemus pheromone-binding proteins with a fluorescence binding assay. J.Biol. Chem. 276, 2007820084.
  • 5
    Pelosi, P. & Maida, R. (1995) Odorant-binding proteins in insects. Comp. Biochem. Physiol. 111B, 503514.
  • 6
    Kim, M.S., Repp, A. & Smith, D.P. (1998) LUSH odorant-binding protein mediates chemosensory responses to alcohols in Drosophila melanogaster. Genetics 150, 711721.
  • 7
    Kim, M.S. & Smith, D.P. (2001) The invertebrate odorant-binding protein LUSH is required for normal olfactory behavior in Drosophila. Chem. Senses 26, 195199.
  • 8
    Wang, Y., Wright, N.J., Guo, H., Xie, Z., Svoboda, K., Malinow, R., Smith, D.P. & Zhong, Y. (2001) Genetic manipulation of the odor-evoked distributed neural activity in the Drosophila mushroom body. Neuron 29, 267276.
  • 9
    Carlson, J.R. (2001) Viewing odors in the mushroom body of the fly. Trends Neurosci. 24, 497498.
  • 10
    Krieger, M.J. & Ross, K.G. (2002) Identification of a major gene regulating complex social behavior. Science 295, 328332.
  • 11
    McKenna, M.P., Hekmat-Scafe, D.S., Gaines, P. & Carlson, J.R. (1994) Putative Drosophila pheromone-binding proteins expressed in a subregion of the olfactory system. J. Biol. Chem. 269, 1634016347.
  • 12
    Pikielny, C.W., Hasan, G., Rouyer, F. & Rosbash, M. (1994) Members of a family of Drosophila putative odorant-binding proteins are expressed in different subsets of olfactory hairs. Neuron 12, 3549.
  • 13
    Ozaki, M., Morisaki, K., Idei, W., Ozaki, K. & Tokunaga, F. (1995) A putative lipophilic stimulant carrier protein commonly found in the taste and olfactory systems. A unique member of the pheromone-binding protein superfamily. Eur. J. Biochem. 230, 298308.
  • 14
    Maleszka, R. & Stange, G. (1997) Molecular cloning, by a novel approach, of a cDNA encoding a putative olfactory protein in the labial palps of the moth Cactoblastis cactorum. Gene 202, 3943.
  • 15
    Bohbot, J., Sobrio, F., Lucas, P. & Nagnan-Le Meillour, P. (1998) Functional characterization of a new class of odorant-binding proteins in the moth Mamestra brassicae. Biochem. Biophys. Res. Commun. 253, 489494.
  • 16
    Picimbon, J.-F., Dietrich, K., Angeli, S., Scaloni, A., Krieger, J., Breer, H. & Pelosi, P. (2000) Purification and molecular cloning of chemosensory proteins from Bombyx mori. Arch. Insect Biochem. Physiol. 44, 120129.
  • 17
    Nagnan-Le Meillour, P., Cain, A.H., Jacquin-Joly, E., François, M.C., Ramachandran, S., Maida, R. & Steinbrecht, R.A. (2000) Chemosensory proteins from the proboscis of Mamestra brassicae. Chem. Senses 25, 541553.
  • 18
    Jacquin-Joly, E., Vogt, R.G., Francois, M.C. & Nagnan-Le Meillour, P. (2001) Functional and expression pattern analysis of chemosensory proteins expressed in antennae and pheromonal gland of Mamestra brassicae. Chem. Senses 26, 833844.
  • 19
    Picimbon, J.F., Dietrich, K., Krieger, J. & Breer, H. (2001) Identity and expression pattern of chemosensory proteins in Heliothis virescens (Lepidoptera, Noctuidae). Insect Biochem. Mol. Biol. 31, 11731181.
  • 20
    Danty, E., Arnold, G., Huet, J.-C., Huet, D., Masson, C. & Pernollet, J.-C. (1998) Separation, characterization and sexual heterogeneity of multiple putative odorant-binding proteins in the honeybee Apis mellifera L. (Hymenoptera: Apidea). Chem. Senses 23, 8391.
  • 21
    Wojtasek, H., Hansson, B.S. & Leal, W.S. (1998) Attracted or repelled? a matter of two neurons, one pheromone binding protein, and a chiral center. Biochem. Biophys. Res. Commun. 250, 217222.
  • 22
    Picimbon, J.-F. & Leal, W.S. (1999) Olfactory soluble proteins of cockroaches. Insect Biochem. Molec. Biol. 29, 973978.
  • 23
    Angeli, S., Ceron, F., Scaloni, A., Monti, M., Monteforti, G., Minnocci, A., Petacchi, R. & Pelosi, P. (1999) Purification, structural characterization, cloning and immunocytochemical localization of chemoreception proteins from Schistocerca gregaria. Eur. J. Biochem. 262, 745754.
  • 24
    Picimbon, J.-F., Dietrich, K., Breer, H. & Krieger, J. (2000) Chemosensory proteins of Locusta migratoria (Orthoptera: Acrididae). Insect Biochem. Molec. Biol. 30, 233241.
  • 25
    Tuccini, A., Maida, R., Rovero, P., Mazza, M. & Pelosi, P. (1996) Putative odorant-binding protein in antennae and legs of Carausius morosus (Insecta, Phasmatodea). Insect Biochem. Molec. Biol. 1, 1924.
  • 26
    Mameli, M., Tuccini, A., Mazza, M., Petacchi, R. & Pelosi, P. (1996) Soluble proteins in chemosensory organs of phasmids. Insect Biochem. Mol. Biol. 26, 875882.
  • 27
    Marchese, S., Angeli, S., Andolfo, A., Scaloni, A., Brandazza, A., Mazza, M., Picimbon, J.-F., Leal, W.S. & Pelosi, P. (2000) Soluble proteins from chemosensory organs of Eurycantha calcarata (Insects, Phasmatodea). Insect Biochem. Mol. Biol. 30, 10911098.
  • 28
    Nomura, A., Kawasaki, K., Kubo, T. & Natori, S. (1992) Purification and localization of p10, a novel protein that increases in nymphal regenerating legs of Periplaneta americana (American cockroach). Int. J. Dev. Biol. 36, 391398.
  • 29
    Kitabayashi, A.N., Arai, T., Kubo, T. & Natori, S. (1998) Molecular cloning of cDNA for p10, a novel protein that increases in the regenerating legs of Periplaneta americana (American cockroach). Insect Biochem. Mol. Biol. 28, 785790.
  • 30
    Dyanov, H.M. & Dzitoeva, S.G. (1995) Method for attachment of microscopic preparations on glass for in situ hybridization, PRINS and in situ PCR studies. Biotechniques 18, 822824.
  • 31
    Picone, D., Crescenzi, O., Angeli, S., Marchese, S., Brandazza, A., Ferrara, L., Pelosi, P. & Scaloni, A. (2001) Bacterial expression and conformational analysis of a chemosensory protein from Schistocerca gregaria. Eur. J. Biochem. 268, 47944801.
  • 32
    Lartigue, A., Campanacci, V., Roussel, A., Larsson, A.M., Jones, T.A., Tegoni, M. & Cambillau, C. (2002) X-Ray structure and ligand binding study of a moth chemosensory protein. J. Biol. Chem. 277, 3209432098.
  • 33
    Campanacci, V., Spinelli, S., Lartigue, A., Lewandowsky, C., Brown, K., Tegoni, M. & Cambillau, C. (2001) Recombinant chemosensory protein (CSP2) from the moth Mamestra brassicae: crystallization and preliminary crystallographic study. Acta Cryst. D57, 137139.
  • 34
    Campanacci, V., Mosbah, A., Bornet, O., Wechselberger, R., Jacquin-Joly, E., Cambillau, C., Darbon, H. & Tegoni, M. (2001) Chemosensory protein from the moth Mamestra brassicae. Expression and secondary structure from 1H and 15N NMR. Eur. J. Biochem. 268, 47314739.
  • 35
    Hildebrand, J.G. & Shepherd, G.M. (1997) Mechanisms of olfactory discrimination: converging evidence for common principles across phyla. Annu. Rev. Neurosci. 20, 595631.
  • 36
    Laska, M., Galizia, C.G., Giurfa, M. & Menzel, R. (1999) Olfactory discrimination ability and odor structure-activity relationships in honeybees. Chem. Senses 24, 429438.
  • 37
    Vogt, R.G., Callahan, F.E., Rogers, M.E. & Dickens, J.C. (1999) Odorant binding protein diversity and distribution among the insect orders, as indicated by LAP, an OBP-related protein of the true bug Lygus lineolaris (Hemiptera, Heteroptera). Chem. Senses 24, 481495.
  • 38
    Danty, E., Michard-Vanhée, C., Huet, J.-C., Genecque, E., Pernollet, J.-C. & Masson, C. (1997) Biochemical characterization, molecular cloning and localization of a putative odorant-binding protein in the honey bee Apis mellifera L. (Hymenoptera: Apidea). FEBS Lett. 414, 595598.
  • 39
    Danty, E., Briand, L., Michard-Vanhée, C., Perez, V., Arnold, G., Gaudemer, O., Huet, D., Huet, J.-C., Ouali, C., Masson, C. & Pernollet, J.-C. (1999) Cloning and expression of a queen pheromone-binding protein in the honeybee: an olfactory-specific, developmentally regulated protein. J. Neuroscience 19, 74687475.
  • 40
    Free, J.B. (1987) Pheromones of Social Bees. Chapman & Hall, London.
  • 41
    Brockmann, A., Brückner, D. & Crewe, R.M. (1998) The EAG response spectra of workers and drones to queen honeybee mandibular gland components: the evolution of a social signal. Naturwissenschaften 85, 283285.
  • 42
    Briand, L., Nespoulous, C., Huet, J.-C., Takahashi, M. & Pernollet, J.-C. (2001) Ligand binding and physico-chemical properties of ASP2, a recombinant odorant-binding protein from honeybee (Apis mellifera L.). Eur. J. Biochem. 268, 752760.
  • 43
    Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 46734680.
  • 44
    Briand, L., Perez, V., Huet, J.-C., Danty, E., Masson, C. & Pernollet, J.-C. (1999) Optimization of the production of a honeybee odorant-binding protein by Pichia pastoris. Prot. Expr. Purif. 15, 362369.
  • 45
    Sallantin, M., Huet, J.-C., Demarteau, C. & Pernollet, J.-C. (1990) Reassessment of commercially available molecular weight standards for peptide sodium dodecyl sulfate-polyacrylamide gelelectrophoresis using electroblotting and microsequencing. Electrophoresis 11, 3436.
  • 46
    Pace, C.N., Vajdos, F., Fee, L., Grimsley, G. & Gray, T. (1995) How to measure and predict the molar absorption coefficient of a protein. Protein Sci. 4, 24112423.
  • 47
    Deleage, G. & Geourjon, C. (1993) An interactive graphic program for calculating the secondary structure content of proteins from circular dichroism spectrum. Comput. Applic. Biosci. 9, 197199.
  • 48
    Briand, L., Nespoulous, C., Huet, J.-C. & Pernollet, J.-C. (2001) Disulfide pairing and secondary structure of ASP1, an olfactory-binding protein from honeybee (Apis mellifera L). J. Peptide Res. 58, 540545.
  • 49
    Norris, A.W. & Li, E. (1998) Retinoid protocols. In Methods in Molecular Biology, (Redfern, C.P.F., eds), Vol. 89, pp. 123139. Humana Press Inc, Totowa, NJ, USA
  • 50
    Slessor, K.N., Kalinski, L.-A., King, G.G.S., Borden, J.H. & Winston, M.L. (1988) Semiochemicals basis of the retinue response to queen honey bees. Nature 332, 354356.
  • 51
    Briand, L., Nespoulous, C., Perez, V., Rémy, J.-J., Huet, J.-C. & Pernollet, J.-C. (2000) Ligand-binding properties and structural characterization of a novel rat odorant-binding protein variant. Eur. J. Biochem. 267, 30793089.
  • 52
    Ramoni, R., Vincent, F., Grolli, S., Conti, V., Malosse, C., Boyer, F.D., Nagnan-Le Meillour, P., Spinelli, S., Cambillau, C. & Tegoni, M. (2001) The insect attractant 1-octen-3-ol is the natural ligand of bovine odorant-binding protein. J. Biol. Chem. 276, 71507155.
  • 53
    Jain, M.K. & Maliwal, B.P. (1985) The environment of tryptophan in pig pancreatic phospholipase A2 bound to bilayers. Biochim. Biophys. Acta 814, 135140.
  • 54
    de Foresta, B., Champeil, P. & Le Maire, M. (1990) Different classes of tryptophan residues involved in the conformational changes characteristic of the sarcoplasmic reticulum Ca2(+)-ATPase cycle. Eur. J. Biochem. 194, 383388.
  • 55
    Mei, B., Kennedy, M.W., Beauchamp, J., Komuniecki, P.R. & Komuniecki, R. (1997) Secretion of a novel, developmentally regulated fatty acid-binding protein into the perivitelline fluid of the parasitic nematode, Ascaris suum. J. Biol. Chem. 272, 99339941.
  • 56
    Lechner, M., Wojnar, P. & Redl, B. (2001) Human tear lipocalin acts as an oxidative-stress-induced scavenger of potentially harmful lipid peroxidation products in a cell culture system. Biochem. J. 356, 129135.
  • 57
    Le Conte, Y., Arnold, G., Trouiller, J. & Masson, C. (1990) Identification of a brood pheromone in honeybees. Naturwissenschaften 77, 334336.
  • 58
    Mohammedi, A., Paris, A., Crauser, D. & Le Conte, Y. (1998) Effect of alphatic esters on ovary development of queenless bees (Apis mellifera L.). Naturwissenschaften 83, 455458.
  • 59
    Knudsen, J.T., Tollsten, L. & Bergström, L.G. (1993) Floral scents – a checklist of volatile compounds isolated by head-space techniques. Phytochemistry 33, 253280.
  • 60
    Salvy, M., Martin, C., Bagneres, A.G., Provost, E., Roux, M., Le Conte, Y. & Clement, J.-L. (2001) Modifications of the cuticular hydrocarbon profile of Apis mellifera worker bees in the presence of the ectoparasitic mite Varroa jacobsoni in brood cells. Parasitology 122, 145159.
  • 61
    Ban, L., Zhang, L., Yan, Y. & Pelosi, P. (2002) Binding properties of a locust's chemosensory protein. Biochem. Biophys. Res. Commun. 293, 5054.
  • 62
    Matayoshi, E.D. & Kleinfeld, A.M. (1981) Emission wavelength-dependent decay of the 9-anthroyloxy-fatty acid membrane probes. Biophys. J. 352, 212235.
  • 63
    Douliez, J.P., Michon, T. & Marion, D. (2000) Steady-state tyrosine fluorescence to study the lipid-binding properties of a wheat non-specific lipid-transfer protein (nsLTP1). Biochim. Biophys. Acta 1467, 6572.
  • 64
    Curry, S., Brick, P. & Franks, N.P. (1999) Fatty acid binding to human serum albumin: new insights from crystallographic studies. Biochim. Biophys. Acta 1441, 131140.
  • 65
    Kowcun, A., Honson, N. & Plettner, E. (2001) Olfaction in the gypsy moth, Lymantria dispar: effect of pH, ionic strength, and reductants on pheromone transport by pheromone-binding proteins. J. Biol. Chem. 276, 4477044776.