• 1
    Zasloff, M. (2002) Antimicrobial peptides of multicellular organisms. Nature (London) 415, 389395.
  • 2
    Hancock, R.E. & Scott, M.G. (2000) The role of antimicrobial peptides in animal defenses. Proc. Natl. Acad. Sci. USA 97, 88568861.
  • 3
    Hancock, R.E. & Diamond, G. (2000) The role of cationic antimicrobial peptides in innate host defences. Trends Microbiol. 8, 402410.
  • 4
    Evelyn, T.P.T. (1996) Infection and disease. In The Fish Immune System: Organism, Pathogen, and Environment (Iwama, G. & Nakanishi, T., eds), pp. 339366. Academic Press, Inc, San Diego.
  • 5
    Yano, T. (1996) The nonspecific immune system: humoral defense. In The Fish Immune System: Organism, Pathogen, and Environment (Iwama, G. & Nakanishi, T., eds), pp. 105157. Academic Press, Inc, San diego.
  • 6
    Lemaitre, C., Orange, N., Saglio, P., saint, N., Gagnon, J. & Molle, G. (1996) Characterization and ion channel activities of novel antibacterial proteins from skin mucosa of carp (Cyprinus carpio). Eur. J. Biochem. 240, 143149.
  • 7
    Robinette, D., Wada, S., Arroll, T., Levy, M.G., Miller, W.L. & Noga, E.J. (1998) Antimicrobial activity in the skin of the channel catfish Ictalurus punctatus: characterization of broad-spectrum histone-like antimicrobial proteins. CMLS, Cell. Mol. Life Sci. 54, 467475.
  • 8
    Tossi, A., Sandri, L. & Giangaspero, A. (2000) Amphipathic, alpha-helical antimicrobial peptides. Biopolymers 55, 430.
  • 9
    Robinette, D.W. & Noga, E.J. (2001) Histone-like protein: a novel method for measuring stress in fish. Diseases of Aquatic Organisms 44, 97107.
  • 10
    Richards, R.C., O'Neil, D.B., Thibault, P. & Ewart, K.V. (2001) Histone H1: an antimicrobial protein of Atlantic salmon (Salmo salar). Biochem. Biophys. Res. Commun. 284, 549555.
  • 11
    Oren, Z. & Shai, Y. (1996) A class of highly potent antibacterial peptides derived from pardaxin, a pore-forming peptide isolated from moses sole fish Pardachirus marmoratus. Eur. J. Biochem. 237, 303310.
  • 12
    Cole, A.M., Weis, P. & Diamond, G. (1997) Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder. J. Biol. Chem. 272, 1200812013.
  • 13
    Park, I.Y., Park, C.B., Kim, M.S. & Kim, S.C. (1998) Parasin I, an antimicrobial peptide derived from histone H2A in the catfish, Parasilurus asotus. FEBS Lett. 437, 258262.
  • 14
    Douglas, S.E., Gallant, J.W., Gong, Z. & Hew, C. (2001) Cloning and developmental expression of a family of pleurocidin-like antimicrobial peptides from winter flounder, Pleuronectes americanus (Walbaum). Dev. Comp. Immunol. 25, 137147.
  • 15
    Park, C.B., Lee, J.H., Park, I.Y., Kim, M.S. & Kim, S.C. (1997) A novel antimicrobial peptide from the loach, Misgurnus anguillicaudatus. FEBS Lett. 411, 173178.
  • 16
    Silphaduang, U. & Noga, E.G. (2001) Peptide antibiotics in mast cells of fish. Nature (London) 414, 268269.
  • 17
    Lauth, X., Shike, H., Burns, J.C., Westerman, M.E., Ostland, V.E., Carlberg, J.M., Van Olst, J.C., Nizet, V., Taylor, S.W., Shimizu, C. & Bulet, P. (2002) Discovery and characterization of two isoforms of moronecidin, a novel antimicrobial peptide from hybrid striped bass. J. Biol. Chem. 277, 50305039.
  • 18
    Shike, H., Lauth, X., Westerman, M.E., Ostland, V.E., Carlberg, J.M., Van Olst, J.C., Shimizu, C., Bulet, P. & Burns, J.C. (2002) Bass hepcidin is a novel antimicrobial peptide induced by bacterial challenge. Eur. J. Biochem. 269, 22322237.
  • 19
    Zeng, R., Xu, Q., Shao, X.-X., Wang, K.-Y. & Xia, Q.-C. (1999) Characterization and analysis of a novel glycoprotein from snake venom using liquid chromatography-electrospray mass spectrometry and edman degradation. Eur. J. Biochem. 266, 352358.
  • 20
    Fields, G.B. & Noble, R.L. (1990) Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids. Int. J. Pept. Protein Res. 35, 161214.
  • 21
    Noda, M., Yamaguchi, M., Ando, E., Takeda, K. & Nokihara, K. (1994) Synthesis of 5-{[(R,S)-5-[(9-fluorenylmethoxycarbonyl) amino]-10,11-dihydrobenzo[alfa,d]cyclopenten-2-yl]valeric acid (CHA) and 5-{[(R,S)-5-[(9-fluorenylmethoxycarbonyl) amino] dibenzo[alfa,d]cyclohepten-2-yl]valeric acid (CHA) handles for the solid-phase synthesis of C-terminal peptide amides under mild conditions. J. Org. Chem. 59, 79657975.
  • 22
    Matsuzaki, K. (1998) Magainins as paradigm for the mode of action of pore forming polypeptides. Biochim. Biophy. Acta 1376, 391400.
  • 23
    Schagger, H. & von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368379.
  • 24
    Gill, S.C. & von Hippel, P.H. (1989) Caluculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182, 319326.
  • 25
    Juban, M.M., Javadpour, M.M. & Barkley, M.D. (1997) Circular dichroism studies of secondary structure of peptides. In Antibacterial Peptide Protocols (Shafer, W.M., ed.), pp. 7384. Humanna Press, Totowa, NJ.
  • 26
    McLean, L.R., Hagaman, K.A., Owen, T.J. & Krstenansky, J.L. (1991) Minimal peptide length for interaction of amphipathic α-helical peptides with phosphatidylcholine liposomes. Biochemistry 30, 3137.
  • 27
    Yomogida, S., Nagaoka, I. & Yamashita, T. (1996) Purification of the 11- and 15-kDa antibacterial polypeptides from guinea pig neutrophils. Arch. Biochem. Biophys. 328, 219226.
  • 28
    Amiche, M., Seon, A., Wroblewski, H. & Nicolas, P. (2000) Isolation of dermatoxin from frog skin, an antibacterial peptide encoded by a novel member of the dermaseptin gene family. Eur. J. Biochem. 267, 45834592.
  • 29
    Uchiyama, S., Fujikawa, Y., Uematsu, K., Matsuda, H., Aida, S. & Iijima, N. (2002) Localization of group IB phospholipase A2 isoform in the gills of the red sea bream, Pagrus (Chrysophrys) major. Comp. Biochem. Physiol. 132B, 671683.
  • 30
    Cole, A.M., Darouiche, R.O., Legarda, D., Connell, N. & Diamond, G. (2000) Characterization of a fish antimicrobial peptide: gene expression, subcellular localization, and spectrum of activity. Antimicrob. Agents Chemother. 44, 20392045.
  • 31
    Taylor, S.W., Craig, A.G., Fischer, W.H., Park, M. & Lehrer, R.I. (2000) Styelin D, an extensively modified antimicrobial peptide from ascidian hemocytes. J. Biol. Chem. 275, 3841738426.
  • 32
    Vogel, H. & Jahnig, F. (1986) The structure of melittin in membranes. Biophys. J. 50, 573582.
  • 33
    Perez-Paya, E., Dufourcq, J., Braco, L. & Abad, C. (1997) Structural characterisation of the natural membrane-bound state of melittin: a fluorescence study of a dansylated analogue. Biochim. Biophys. Acta 1329, 223236.
  • 34
    Ladokhin, A.S. & White, S.T. (1999) Folding amphipathic α-helices on membranes: energetics of helix formation by melittin. J. Mol. Biol. 285, 13631369.
  • 35
    Reite, O.B. (1997) Mast cells/eosinophilic granule cells of salmonids: staining properties and responses to noxious agents. Fish Shellfish Immunol. 7, 567584.
  • 36
    Laurent, P. (1984) Gill internal morphology. In Fish Physiology (Hoar, W.S. & Randall, D.J., eds), pp. 73183. Academic Press, Inc, Orlando.
  • 37
    Goss, G.G., Perry, S.F., Fryer, J.N. & Lauent, P. (1998) Gill morphology and acid-base regulation in freshwater fishes. Comp. Biochem. Physiol. 119A, 107115.