• 1
    Kearsey, S.E. & Labib, K. (1998) MCM proteins: evolution, properties, and role in DNA replication. Biochim. Biophys. Acta 1398, 113136.
  • 2
    Tye, B.K. (1999) MCM proteins in DNA replication. Annu. Rev. Biochem. 68, 649686.
  • 3
    Kelly, T.J. & Brown, G.W. (2000) Regulation of chromosome replication. Annu. Rev. Biochem. 69, 829880.
  • 4
    Bell, S.P. & Dutta, A. (2002) DNA replication in eukaryotic cells. Annu. Rev. Biochem. 71, 333374.
  • 5
    Labib, K., Tercero, J.A. & Diffley, J.F. (2000) Uninterrupted MCM2-7 function required for DNA replication fork progression. Science 288, 16431647.
  • 6
    Tanaka, T., Knapp, D. & Nasmyth, K. (1997) Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs. Cell 90, 649660.
  • 7
    Aparicio, O.M., Weinstein, D.M. & Bell, S.P. (1997) Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. Cell 91, 5969.
  • 8
    Thommes, P., Kubota, Y., Takisawa, H. & Blow, J.J. (1997) The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides. EMBO J. 16, 33123319.
  • 9
    Koonin, E.V. (1993) A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication. Nucleic Acids Res. 21, 25412547.
  • 10
    Ishimi, Y. (1997) A DNA helicase activity is associated with an MCM4,-6, and -7 protein complex. J. Biol. Chem. 272, 2450824513.
  • 11
    You, Z., Komamura, Y. & Ishimi, Y. (1999) Biochemical analysis of the intrinsic Mcm4-Mcm6-Mcm7 DNA helicase activity. Mol. Cell. Biol. 19, 80038015.
  • 12
    Lee, J.-K. & Hurwitz, J. (2000) Isolation and characterization of various complexes of the minichromosome maintenance proteins of Schizosaccharomyces pombe. J. Biol. Chem. 275, 1887118878.
  • 13
    Lee, J.-K. & Hurwitz, J. (2001) Processive DNA helicase activity of the minichromosome maintenance proteins 4, 6, and 7 complex requires forked DNA structures. Proc. Natl Acad. Sci. USA 98, 5459.
  • 14
    Ishimi, Y., Komamura, Y., You, Z. & Kimura, H. (1998) Biochemical function of mouse minichromosome maintenance 2 protein. J. Biol. Chem. 273, 83698375.
  • 15
    Sato, M., Gotow, T., You, Z., Komamura-Kohno, Y., Uchiyama, Y., Yabuta, N., Nojima, H. & Ishimi, Y. (2000) Electron microscopic observation and single-stranded DNA binding activity of the Mcm4,6,7 complex. J. Mol. Biol. 300, 421431.
  • 16
    Kubota, Y., Mimura, S., Nishimoto, S.I., Takisawa, H. & Nojima, H. (1995) Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor. Cell 81, 601609.
  • 17
    Chong, P.J., Mahbubani, H.M., Khoo, C.Y. & Blow, J.J. (1995) Purification of an MCM-containing complex as a component of the DNA replication licensing system. Nature 375, 418421.
  • 18
    Madine, M.A., Khoo, C.Y., Mills, A.D. & Laskey, R.A. (1995) MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells. Nature 375, 421425.
  • 19
    Lygerou, Z. & Nurse, P. (2000) Controlling S-phase onset in fission yeast. Biological responses to DNA damage. Symposium on Quantitative Biology, 65, 323332.
  • 20
    Nguyen, Y.Q., Co, C. & Li, J.J. (2001) Cyclin-dependent kinases prevent DNA re-replication through multiple mechanisms. Nature 411, 10681073.
  • 21
    Brodeur, G.M. & Hogarty, M.D. (1998) Gene amplification in human cancers: biological and clinical significance. In The Genetic Basis of Human Cancer (Vogelstein, B. & Kinzler, K.W., eds), pp 161172. McGraw-Hill, Health Professions Division.
  • 22
    Schimke, R.T. (1988) Gene amplification in cultured cells. J. Biol. Chem. 263, 59895992.
  • 23
    Stark, G.R., Debatisse, M., Giulotto, E. & Wahl, G.M. (1989) Recent progress in understanding mechanisms of mammalian DNA amplification. Cell 57, 901908.
  • 24
    Hiraiwa, A., Fujita, M., Nagasaka, T., Adachi, A., Ohashi, M. & Ishibashi, M. (1997) Immunolocalization of hCDC47 protein in normal and neoplastic human tissues and its relation to growth. Int. J. Cancer 74, 180184.
  • 25
    Hiraiwa, A., Fujita, M., Adachi, A., Ono, H., Nagasaka, T., Matsumoto, Y., Ohashi, M., Tomita, Y. & Ishibashi, M. (1998) Specific distribution patterns of hCDC47 expression in cutaneous diseases. J. Cutan. Pathol. 25, 285290.
  • 26
    Williams, G.H., Romanowski, P., Morris, L., Madine, M., Mills, A.D., Stoeber, K., Marr, J., Laskey, R.A. & Coleman, N. (1998) Improved cervical smear assessment using antibodies against proteins that regulate DNA replication. Proc. Natl Acad. Sci. USA 95, 1493214937.
  • 27
    Todorov, I.T., Werness, B.A., Wang, H.-Q., Buddharaju, L.N., Todorova, P.D., Slocum, H.K., Brooks, J.S. & Huberman, J.A. (1998) HsMCM2/BM28: a novel proliferation marker for human tumors and normal tissues. Laboratory Invest. 78, 7378.
  • 28
    Freeman, A., Morris, L.S., Mills, A.D., Stoeber, K., Laskey, R.A., Williams, G.H. & Coleman, N. (1999) Minichromosome maintenance proteins as biological markers of dysplasia and malignancy. Clin. Cancer Res. 5, 21212132.
  • 29
    Stoeber, K., Halsall, I., Freeman, A., Swinn, R., Doble, A., Morris, L., Coleman, N., Bullock, N., Laskey, R.A., Hales, C.N. & Williams, G.H. (1999) Immunoassay for urothelial cancers that detects DNA replication protein Mcm5 in urine. Lancet 354, 15241525.
  • 30
    Stoeber, K., Tisty, T.D., Happerfield, L., Thomas, G.A., Romanov, S., Bobrow, L., Williams, E.D. & Williams, G.H. (2001) DNA replication licensing and human cell proliferation. J. Cell Sci. 114, 20272041.
  • 31
    Wharton, S.B., Chan, K.K., Anderson, J.R., Stoeber, K. & Williams, G.H. (2001) Replicative Mcm2 protein as a novel proliferation marker in oligodendrogliomas and its relationship to Ki67 labelling index, histological grade and prognosis. Neuropath. Appl. Neurobiol. 27, 305313.
  • 32
    Endl, E., Kausch, I., Baak, M., Knippers, R., Gerdes, J. & Scholzen, T. (2001) The expression of Ki-67, MCM3, and p27 defines distinct subsets of proliferating, resting, and differentiated cells. J. Pathol. 195, 457462.
  • 33
    Tan, D.-F., Huberman, J.A., Hyland, A., Loewen, G.M., Brooks, J.S.J., Beck, A.F., Todorov, I.T. & Bepler, G. (2001) MCM2- a promising marker for premalignant lesions of the lung: a cohort study. BMC Cancer 1, 6.
  • 34
    Kimura, H., Ohtomo, T., Yamaguchi, M., Ishii, A. & Sugimoto, K. (1996) Mouse MCM proteins: complex formation and transportation to the nucleus. Genes Cells 1, 977993.
  • 35
    Kimura, H., Takizawa, N., Nozaki, N. & Sugimoto, K. (1995) Molecular cloning of cDNA encoding mouse Cdc21 and CDC46 homologs and characterization of the products: physical interaction between P1 (MCM3) and CDC46 proteins. Nucleic Acids Res. 23, 20972104.
  • 36
    Fujita, M., Ishimi, Y., Nakamura, H., Kiyono, T. & Tsurumi, T. (2002) Nuclear organization of DNA replication initiation proteins in mammalian cells. J. Biol. Chem. 277, 1035410361.
  • 37
    Fujita, M., Kiyono, T., Hayashi, Y. & Ishibashi, M. (1997) In vivo interaction on of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP. J. Biol. Chem. 272, 1092810935.
  • 38
    Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Vol. 3, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  • 39
    Scholzen, T. & Gerdes, J. (2000) The Ki-67 protein: from the known and the unknown. J. Cell Physiol. 182, 311322.
  • 40
    DeFonseca, C.J., Shu, F. & Zhang, J.J. (2001) Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-γ. Proc. Natl Acad. Sci. USA 98, 30343039.
  • 41
    Yankulov, K., Todorov, I., Romanowski, P., Licatalosi, D., Cilli, K., McCracken, S., Laskey, R. & Bentley, D.L. (1999) MCM proteins are associated with RNA polymerase II holoenzyme. Mol. Cell Biol. 19, 61546163.
  • 42
    Sterner, J.M., Dew-Knight, S., Musahl, C., Kornbluth, S. & Horowitz, J.M. (1998) Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with Mcm7. Mol. Cell Biol. 18, 27482757.
  • 43
    Leone, G., DeGregori, J., Yan, Z., Jakoi, L., Ishida, S., Williams, R.S. & Nevins, J.R. (1998) E2F3 activity is regulated during the cell cycle and is required for the induction of S phase. Genes Dev. 12, 21202130.
  • 44
    Tsuruga, H., Yabuta, N., Hosoya, S., Tamura, K., Endo, Y. & Nojima, H. (1997) HsMCM6: a new member of the human MCM/P1 family encodes a protein homologous to fission yeast Mis5. Genes Cells 2, 381399.
  • 45
    Suzuki, S., Adachi, A., Hiraiwa, A., Ohashi, M., Ishibashi, M. & Kiyono, T. (1998) Cloning and characterization of human MCM7 promoter. Gene 216, 8591.
  • 46
    Ohtani, K., Iwanaga, R., Nakamura, M., Ikeda, M., Yabuta, N., Tsuruga, H. & Nojima, H. (1999) Cell growth-regulated expression of mammalian MCM5 and MCM6 genes mediated by the transcription factor E2F. Oncogene 18, 22992309.
  • 47
    Goodwin, E.C. & DiMaio, D. (2000) Repression of human papillomavirus oncogenes in HeLa cervical carcinoma cells causes the orderly reactivation of dormant tumor suppressor pathways. Proc. Natl Acad. Sci. USA 97, 1251312518.
  • 48
    Gozuacik, D., Murakami, Y., Saigo, K., Chami, M., Mugnier, C., Lagorce, D., Okanoue, T., Urashima, T., Brechot, C. & Paterlini-Brechot, P. (2001) Identification of human cancer-related genes by naturally occurring hepatitis B virus DNA tagging. Oncogene 20, 62336240.
  • 49
    Saha, S., Bardelli, A., Buckhaults, P., Velculescu, V.E., Rago, C., Croix, B.S., Romans, K.E., Choti, M.A., Lengauer, C., Kinzler, K.W. & Vogelstein, B. (2001) A phosphatase associated with metastasis of colorectal cancer. Science 294, 13431346.
  • 50
    Shohet, J.M., Hicks, M.J., Plon, S.E., Burlingame, S.M., Stuart, S., Chen, S.-Y., Brenner, M.K. & Nuchtern, J.G. (2002) Minichromosome maintenance protein MCM7 is a direct target of the MYCN transcription factor in neuroblastoma. Cancer Res. 62, 11231128.