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Molecular characterization and allergenic activity of Lyc e 2 (β-fructofuranosidase), a glycosylated allergen of tomato

  1. Sandra Westphal1,
  2. Daniel Kolarich2,
  3. Kay Foetisch1,
  4. Iris Lauer1,
  5. Friedrich Altmann2,
  6. Amedeo Conti3,
  7. Jesus F. Crespo4,
  8. Julia Rodríguez4,
  9. Ernesto Enrique5,
  10. Stefan Vieths1,
  11. Stephan Scheurer1

Article first published online: 3 MAR 2003

DOI: 10.1046/j.1432-1033.2003.03503.x

Issue

European Journal of Biochemistry

European Journal of Biochemistry

Volume 270, Issue 6, pages 1327–1337, March 2003

Additional Information

How to Cite

Westphal, S., Kolarich, D., Foetisch, K., Lauer, I., Altmann, F., Conti, A., Crespo, J. F., Rodríguez, J., Enrique, E., Vieths, S. and Scheurer, S. (2003), Molecular characterization and allergenic activity of Lyc e 2 (β-fructofuranosidase), a glycosylated allergen of tomato. European Journal of Biochemistry, 270: 1327–1337. doi: 10.1046/j.1432-1033.2003.03503.x

Author Information

  1. 1

    Department of Allergology, Paul-Ehrlich-Institut, Langen, Germany;

  2. 2

    Institute of Chemistry, University of Agriculture, Vienna, Austria;

  3. 3

    CNR-ISPA c/o Bioindustry Park, Colleretto Giacosa, Italy;

  4. 4

    Servicio de Alergia, Hospital Universitario Doce de Octubre, Madrid, Spain;

  5. 5

    Institut Universitari Dexeus, Barcelona, Spain

*S. Scheurer, Paul-Ehrlich-Institut, Department of Allergology, Paul-Ehrlich Str. 51–59, D-63225 Langen, Germany. Fax: + 49 6103 77 1258, Tel.: + 49 6103 77 5200, E-mail: schst@pei.de

Publication History

  1. Issue published online: 3 MAR 2003
  2. Article first published online: 3 MAR 2003
  3. (Received 10 October 2002, revised 8 January 2003, accepted 5 February 2003)

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References

  • 1
    de Martino, M., Novembre, E., Cozza, G., de Marco, A., Bonazza, P. & Vierucci, A. (1988) Sensitivity to tomato and peanut allergens in children monosensitized to grass pollen. Allergy 43, 206–213.
    Direct Link:
  • 2
    Beezhold, D.H., Sussman, G.L., Liss, G.M. & Chang, N.S. (1996) Latex allergy can induce clinical reactions to specific foods. Clin. Exp. Allergy 26, 416–422.
    Direct Link:
  • 3
    Kim, K.T. & Hussain, H. (1999) Prevalence of food allergy in 137 latex-allergic patients. Allergy Asthma Proc. 20, 95–97.
  • 4
    Bleumink, E., Berrens, L. & Young, E. (1967) Studies on the atopic allergen in ripe tomato fruits. II. Further chemical characterization of the purified allergen. Int. Arch. Allergy Appl. Immunol. 31, 25–37.
  • 5
    Bleumink, E., Berrens, L. & Young, E. (1966) Studies on the atopic allergen in ripe tomato fruits. I. Isolation and identification of the allergen. Int. Arch. Allergy Appl. Immunol. 30, 132–145.
  • 6
    Darnowski, D.W., Valenta, R. & Parthasarathy, M.V. (1996) Identification and distribution of profilin in tomato (Lycopersicon esculentum Mill.). Planta (Germany) 198, 158–161.
  • 7
    Aalberse, R.C. (1998) Clinical relevance of carbohydrate allergen epitopes. Allergy 53, 54–57.
    Direct Link:
  • 8
    Foetisch, K., Altmann, F., Haustein, D. & Vieths, S. (1999) Involvement of carbohydrate epitopes in the IgE response of celery- allergic patients. Int. Arch. Allergy Immunol. 120, 30–42.
  • 9
    Tretter, V., Altmann, F., Kubelka, V., Marz, L. & Becker, W.M. (1993) Fucose alpha 1,3-linked to the core region of glycoprotein N-glycans creates an important epitope for IgE from honeybee venom allergic individuals. Int. Arch. Allergy Immunol. 102, 259–266.
  • 10
    Alisi, C., Afferni, C., Iacovacci, P., Barletta, B., Tinghino, R., Butteroni, C., Puggioni, E.M., Wilson, I.B., Federico, R., Schi- nina, M.E., Ariano, R., Di Felice, G. & Pini, C. (2001) Rapid isolation, characterization, and glycan analysis of Cup a 1, the major allergen of Arizona cypress (Cupressus arizonica) pollen. Allergy 56, 978–984.
  • 11
    van Ree, R., Cabanes-Macheteau, M., Akkerdaas, J., Milazzo, J.P., Loutelier-Bourhis, C., Rayon, C., Villalba, M., Koppelman, S., Aalberse, R.C., Rodriguez, R., Faye, L. & Lerouge, P. (2000) Beta (1,2)-xylose and alpha (1,3)-fucose residues have a strong contribution in IgE binding to plant glycoallergens. J. Biol. Chem. 275, 11451–11458.
  • 12
    Mueller, U., Luettkopf, D., Hoffmann, A., Petersen, A., Becker, W.M., Schocker, F., Niggemann, B., Altmann, F., Kolarich, D., Haustein, D. & Vieths, S. (2000) Allergens in native and roasted hazelnuts (Corylus avellana) and their cross-reactivity to pollen. Eur. Food Res. Technol. 212, 2–12.
  • 13
    Priem, B., Gitti, R., Bush, C.A. & Gross, K.C. (1993) Structure of ten free N-glycans in ripening tomato fruit. Arabinose is a constituent of a plant N-glycan. Plant Physiol. 102, 445–458.
  • 14
    Zeleny, R., Altmann, F. & Praznik, W. (1999) Structural characterization of the N-linked oligosaccharides from tomato fruit. Phytochemistry 51, 199–210.
  • 15
    Petersen, A., Vieths, S., Aulepp, H., Schlaak, M. & Becker, W.M. (1996) Ubiquitous structures responsible for IgE cross-reactivity between tomato fruit and grass pollen allergens. J. Allergy Clin. Immunol. 98, 805–815.
  • 16
    Kondo, Y., Urisu, A. & Tokuda, R. (2001) Identification and characterization of the allergens in the tomato fruit by immunoblotting. Int. Arch. Allergy Immunol. 126, 294–299.
  • 17
    Sturm, A. & Chrispeels, M.J. (1990) cDNA cloning of carrot extracellular beta-fructosidase and its expression in response to wounding and bacterial infection. Plant Cell 2, 1107–1119.
  • 18
    Leigh, R.A., Rees, T., Fuller, W.A. & Banfield, J. (1979) The location of acid invertase activity and sucrose in the vacuoles of storage roots of beetroot (Beta vulgaris). Biochem. J. 178, 539–547.
  • 19
    Fahrendorf, T. & Beck, E. (1990) Cytosolic and cell wall-bound acid invertases from leaves in Urtica dioica L. Comparison. Planta 180, 237–244.
  • 20
    Yelle, S., Chetelat, R., Dorais, M., DeVerna, J.W. & Bennet, A.B. (1991) Sink metabolism in tomato fruit. Plant Physiol. 95, 1026–1035.
  • 21
    Ohyama, A., Hirai, M. & Nishimura, S. (1992) A novel cDNA clone for acid invertase in tomato fruit. Jpn. J. Genet. 67, 491–492.
  • 22
    Foetisch, K., Son, D.Y., Altmann, F., Aulepp, H., Conti, A., Haustein, D. & Vieths, S. (2001) Tomato (Lycopersicon esculentum) allergens in pollen-allergic patients. Eur. Food. Res. Technol. 213, 259–266.
  • 23
    Vieths, S., Schoning, B. & Petersen, A. (1994) Characterization of the 18-kDa apple allergen by two-dimensional immunoblotting and microsequencing. Int. Arch. Allergy Immunol. 104, 399–404.
  • 24
    Wilson, I.B.H., Harthill, J.E., Mullin, N.P., Ashford, D.A. & Altmann, F. (1998) Core alpha1,3-fucose is a key part of the epitope recognized by antibodies reacting against plant N-linked oligosaccharides and is present in a wide variety of plant extracts. Glycobiology 8, 651–661.
  • 25
    Vieths, S., Janek, K., Aulepp, H. & Petersen, A. (1995) Isolation and characterization of the 18-kDa major apple allergen and comparison with the major birch pollen allergen (Bet v, I). Allergy 50, 421–430.
    Direct Link:
  • 26
    Holzhauser, T. & Vieths, S. (1999) Indirect competitive ELISA for determination of traces of peanut (Arachis hypogaea L.) protein in complex food matrices. J. Agric. Food Chem. 47, 603–611.
  • 27
    Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
  • 28
    Katayama, H., Nagasu, T. & Oda, Y. (2001) Improvement ofin-gel digestion protocol for peptide mass fingerprinting by matrix-assisted laser desorption/ionization time-of- flight mass spectrometry. Rapid Commun. Mass Spectrom. 15, 1416–1421.
    Direct Link:
  • 29
    Kolarich, D. & Altmann, F. (2000) N-Glycan analysis by matrix-assisted laser Desorption/Ionization mass spectrometry of electro- phoretically separated nonmammalian proteins: application to peanut allergen ara h 1 and olive pollen allergen ole e 1. Anal. Biochem. 285, 64–75.
  • 30
    Kleine Budde, I., de Heer, P.G., van der Zee, J.S. & Aalberse, R.C. (2001) The stripped basophil histamine release bioassay as a tool for the detection of allergen-specific IgE in serum. Int. Arch. Allergy Immunol. 126, 277–285.
  • 31
    Pruzansky, J.J., Grammer, L.C., Patterson, R. & Roberts, M. (1983) Dissociation of IgE from receptors on human basophils. I. Enhanced passive sensitization for histamine release. J. Immunol. 131, 1949–1953.
  • 32
    Aalberse, R.C. & van Ree, R. (1996) Cross-reactive carbohydrate determinants. In Highlights in Food Allergy (Wüthrich, B. & Ortolani, C., eds), pp. 78–83. Karger, Basel.
  • 33
    Sturm, A. (1991) Heterogeneity of the complex N-linked oligosaccharides at specific glycosylation sites of two secreted carrot glycoproteins. Eur. J. Biochem. 199, 169–179.
    Direct Link:
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