Enzymes: deoxyhypusine hydroxylase (EC 184.108.40.206); deoxyhypusine synthase (EC 220.127.116.11).
Identification and characterization of eukaryotic initiation factor 5A-2
Article first published online: 1 OCT 2003
European Journal of Biochemistry
Volume 270, Issue 21, pages 4254–4263, November 2003
How to Cite
Clement, P. M. J., Henderson, C. A., Jenkins, Z. A., Smit-McBride, Z., Wolff, E. C., Hershey, J. W. B., Park, M. H. and Johansson, H. E. (2003), Identification and characterization of eukaryotic initiation factor 5A-2. European Journal of Biochemistry, 270: 4254–4263. doi: 10.1046/j.1432-1033.2003.03806.x
Present address: Laboratory of Oncology, Catholic University of Leuven, Belgium.
† Present address: Department Paediatrics and Child Health, Dunedin School of Medicine, The University of Otago, New Zealand.
- Issue published online: 16 OCT 2003
- Article first published online: 1 OCT 2003
- (Received 23 June 2003, revised 20 August 2003, accepted 26 August 2003)
- post-translational modification
The phylogenetically conserved eukaryotic translation initiation factor 5A (eIF5A) is the only known cellular protein to contain the post-translationally derived amino acid hypusine [Nε-(4-amino-2-hydroxybutyl)lysine]. Both eIF5A and its hypusine modification are essential for sustained cell proliferation. Normally only one eIF5A protein is expressed in human cells. Recently, we identified a second human EIF5A gene that would encode an isoform (eIF5A-2) of 84% sequence identity. Overexpression of eIF5A-2 mRNA in certain human cancer cells, in contrast to weak normal expression limited to human testis and brain, suggests EIF5A2 as a potential oncogene. However, eIF5A-2 protein has not been described in human or mammalian cells heretofore. Here, we describe the identification of eIF5A-2 protein in human colorectal and ovarian cancer lines, SW-480 and UACC-1598, that overexpress eIF5A-2 mRNAs. Functional characterization of the human isoforms revealed that either human EIF5A gene can complement growth of a yeast strain in which the yeast EIF5A genes were disrupted. This indicates functional similarity of the human isoforms in yeast and suggests that eIF5A-2 has an important role in eukaryotic cell survival similar to that of the ubiquitous eIF5A-1. Detectable structural differences were also noted, including lack of immunological cross-reactivity, formation of different complexes with deoxyhypusine synthase, and Km values (1.5 ± 0.2 vs. 8.3 ± 1.4 µm for eIF5A-1 and -2, respectively) as substrates for deoxyhypusine synthase in vitro. These physical characteristics and distinct amino acid sequences in the C-terminal domain together with differences in gene expression patterns imply differentiated, tissue-specific functions of the eIF5A-2 isoform in the mammalian organism and in cancer.