Correspondence to G. Widmalm, Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-106 91 Stockholm, Sweden
Structural determination of the O-antigenic polysaccharide from Escherichia coli O35 and cross-reactivity to Salmonella arizonae O62
Article first published online: 25 DEC 2001
European Journal of Biochemistry
Volume 258, Issue 1, pages 139–143, November (II) 1998
How to Cite
Rundlöf, T., Weintraub, A. and Widmalm, G. (1998), Structural determination of the O-antigenic polysaccharide from Escherichia coli O35 and cross-reactivity to Salmonella arizonae O62. European Journal of Biochemistry, 258: 139–143. doi: 10.1046/j.1432-1327.1998.2580139.x
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Abbreviations. DQF, double quantum filtered; EIA, enzyme immunoassay; GPC, gel-permeation chromatography; HMBC, heteronuclear multiple bond connectivity; HSQC, heteronuclear single quantum coherence; HMQC, heteronuclear multiple quantum coherence; LPS, lipopolysaccharide; Rha, rhamnose, 6-deoxymannose; TSP, 3-(trimethylsilyl)-[2,2,3,3-2H4]propanoate.
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
- (Received 8 June/25 August 1998)
- Cited By
The structure of the O-antigenic polysaccharide from Escherichia coli O35 has been investigated with the aid of NMR spectroscopy, sugar and methylation analyses. The sequence of the sugar residues could be determined by NOESY and heteronuclear-multiple-bond-connectivity NMR experiments. The polysaccharide is composed of hexasaccharide repeating units with the following structure, where Rha and GalNAcAN represent rhamnose and 2-acetamido-2-deoxy-galacturonamide, respectively : 3)-β- D-GlcpNAc-(13)-α- L-Rhap-(12)-α- L-Rhap-(13)-α- L-Rhap-(12)-α- L-Rhap-(1
The O-antigen of Escherichia coli O35 is similar to the O-specific polysaccharide from Salmonella arizonae O62, which instead has a terminal 2-acetamido-2-deoxy-α- D-galacturonic acid residue. Immunochemical analyses using a rabbit antiserum specific for the Salmonella arizonae O62 O-antigen showed an identical reactivity with both lipopolysaccharides.