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Desulfovibrio gigas neelaredoxin

A novel superoxide dismutase integrated in a putative oxygen sensory operon of an anaerobe

  1. Gabriela Silva1,2,
  2. Solange Oliveira1,3,
  3. Cláudio M. Gomes2,
  4. Isabel Pacheco2,
  5. Ming Y. Liu4,
  6. António V. Xavier2,
  7. Miguel Teixeira2,
  8. Jean LeGall2,4,
  9. Claudina Rodrigues-Pousada1,2

Article first published online: 25 DEC 2001

DOI: 10.1046/j.1432-1327.1999.00025.x

Issue

European Journal of Biochemistry

European Journal of Biochemistry

Volume 259, Issue 1-2, pages 235–243, January 1999

Additional Information

How to Cite

Silva, G., Oliveira, S., Gomes, C. M., Pacheco, I., Liu, M. Y., Xavier, A. V., Teixeira, M., LeGall, J. and Rodrigues-Pousada, C. (1999), Desulfovibrio gigas neelaredoxin. European Journal of Biochemistry, 259: 235–243. doi: 10.1046/j.1432-1327.1999.00025.x

Author Information

  1. 1

    Instituto Gulbenkian de Ciência, Oeiras, Portugal;

  2. 2

    Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal;

  3. 3

    Universidade Évora, Departamento de Biologia, Évora, Portugal;

  4. 4

    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA

* C. Rodrigues-Pousada, Instituto Gulbenkian de Ciência, 2781 Oeiras, Portugal. Tel. +351-1-4407900, Fax: +351-1-4407970, E-mail: claudrod@pen.gulbenkian.pt

Publication History

  1. Issue published online: 25 DEC 2001
  2. Article first published online: 25 DEC 2001
  3. (Received 20 July 1998; accepted 13 October 1998)

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Keywords:

  • anaerobes;
  • oxygen-sensing mechanisms;
  • sulfate-reducing bacteria;
  • superoxide dismutase

Neelaredoxin, a small non-heme blue iron protein from the sulfate-reducing bacterium Desulfovibrio gigas[Chen, L., Sharma, P., LeGall, J., Mariano, A.M., Teixeira M. and Xavier, A.V. (1994) Eur. J. Biochem.226, 613–618] is shown to be encoded by a polycistronic unit which contains two additional open reading frames (ORF-1 and ORF-2) coding for chemotaxis-like proteins. ORF-1 has domains highly homologous with those structurally and functionally important in methyl-accepting chemotaxis proteins, including two putative transmembrane helices, potential methylation sites and the interaction domain with CheW proteins. Interestingly, ORF-2 encodes a protein having homologies with CheW proteins.

 Neelaredoxin is also shown to have significant superoxide dismutase activity (1200 U·mg–1), making it a novel type of iron superoxide dismutase. Analysis of genomic data shows that neelaredoxin-like putative polypeptides are present in strict anaerobic archaea, suggesting that this is a primordial superoxide dismutase.

 The three proteins encoded in this operon may be involved in the oxygen-sensing mechanisms of this anaerobic bacterium, indicating a possible transcriptional mechanism to sense and respond to potential stress agents.

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