You have full text access to this OnlineOpen article

Purification, characterization and structural analysis of an abundant β-1,3-glucanase from banana fruit

  1. Willy J. Peumans1,
  2. Annick Barre2,
  3. Veerle Derycke3,
  4. Pierre Rougé2,
  5. Wenling Zhang1,
  6. Gregory D. May4,
  7. Jan A. Delcour3,
  8. Fred Van Leuven5,
  9. Els J. M. Van Damme1

Article first published online: 25 DEC 2001

DOI: 10.1046/j.1432-1327.2000.01117.x

Issue

European Journal of Biochemistry

European Journal of Biochemistry

Volume 267, Issue 4, pages 1188–1195, February 2000

Additional Information

How to Cite

Peumans, W. J., Barre, A., Derycke, V., Rougé, P., Zhang, W., May, G. D., Delcour, J. A., Van Leuven, F. and Van Damme, E. J. M. (2000), Purification, characterization and structural analysis of an abundant β-1,3-glucanase from banana fruit. European Journal of Biochemistry, 267: 1188–1195. doi: 10.1046/j.1432-1327.2000.01117.x

Author Information

  1. 1

    Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Belgium;

  2. 2

    Institut de Pharmacologie et Biologie Structurale, UPR CNRS 9062, Toulouse, France;

  3. 3

    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Belgium;

  4. 4

    Plant Biology Division, The Samuel Roberts Noble Foundation Inc., Ardmore, OK, USA;

  5. 5

    Center for Human Genetics, Katholieke Universiteit Leuven, Belgium,

* W. Peumans, Katholieke Universiteit Leuven, Laboratory for Phytopathology and Plant Protection, Willem de Croylaan 42, 3001 Leuven, Belgium. E-mail: Willy.Peumans@agr.kuleuven.ac.be

Publication History

  1. Issue published online: 25 DEC 2001
  2. Article first published online: 25 DEC 2001
  3. (Received 2 November 1999, revised 16 December 1999, accepted 21 December 1999)

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (1378K)

Keywords:

  • banana;
  • β-1;
  • 3-glucanase;
  • Musa;
  • PR protein

An abundant, catalytically active β-1,3-endoglucanase (EC 3.2.1.39) has been isolated from the pulp of ripe bananas. Biochemical analysis of the purified protein, molecular modelling, and molecular cloning of the corresponding gene indicate that this banana enzyme closely resembles previously characterized plant β-glucanases with respect to its amino-acid sequence, structure and biological activity. The results described in this paper demonstrate both the occurrence of an abundant active β-1,3-endoglucanases in fruits and also readdress the question of the possible involvement of these enzymes in the ripening and/or softening process.

View Full Article (HTML) Get PDF (1378K)