Present address: Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA.
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Concomitant induction of apoptosis and expression of monocyte chemoattractant protein-1 in cultured rat luteal cells by nuclear factor-κB and oxidative stress
Article first published online: 3 SEP 2003
DOI: 10.1046/j.1440-169X.2003.00704.x
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How to Cite
Nagaosa, K., Shiratsuchi, A. and Nakanishi, Y. (2003), Concomitant induction of apoptosis and expression of monocyte chemoattractant protein-1 in cultured rat luteal cells by nuclear factor-κB and oxidative stress. Development, Growth & Differentiation, 45: 351–359. doi: 10.1046/j.1440-169X.2003.00704.x
Publication History
- Issue published online: 3 SEP 2003
- Article first published online: 3 SEP 2003
- Received 10 February 2003; revised 22 May 2003; accepted 3 June 2003.
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Keywords:
- apoptosis;
- chemokine expression;
- corpus luteum;
- nuclear factor (NF)-κB;
- oxidative stress
It has previously been shown that expression of monocyte chemoattractant protein (mcp)-1 and apoptosis of luteal cells occur concomitantly during the estrous cycle in the rat corpus luteum; however, luteal cells containing mcp-1 mRNA did not seem to be apoptotic. In the present study, the relationship between the induction of apoptosis and mcp-1 expression in cultures of dispersed rat luteal cells was examined. Both apoptosis and mcp-1 expression were spontaneously induced in cultured luteal cells in a manner inhibitable by antioxidative reagents or an inhibitor of nuclear translocation of nuclear factor-kB. However, the cells containing mcp-1 mRNA were distinct from those undergoing apoptosis, and the inhibition of apoptosis by the pan-caspase inhibitor z-VAD-fmk did not influence the induction of mcp-1 expression. These results collectively indicate that oxidative stress simultaneously, but independently, induces apoptosis and mcp-1 expression in luteal cells through the activation of nuclear factor-kB. This phenomenon might help to explain how monocytes/macrophages accumulate in regressive corpora lutea where their target apoptotic cells exist.