DNA vaccination against hepatitis E virus infection in cynomolgus macaques
Article first published online: 23 SEP 2008
Journal of Gastroenterology and Hepatology
Volume 17, Issue Supplement s3, pages S365–S369, December 2002
How to Cite
KAMILI, S., SPELBRING, J. and KRAWCZYNSKI, K. (2002), DNA vaccination against hepatitis E virus infection in cynomolgus macaques. Journal of Gastroenterology and Hepatology, 17: S365–S369. doi: 10.1046/j.1440-1746.17.s3.29.x
- Issue published online: 23 SEP 2008
- Article first published online: 23 SEP 2008
- DNA vaccines;
- hepatitis E virus;
- HEV ORF2;
- IgG anti-HEV
Background : The feasibility of DNA vaccination against hepatitis E in non-human primates has not been evaluated. In the present study a full-length hepatitis E virus (HEV) open reading frame (ORF)2 (Burmese strain) was assembled, cloned, and used for genetic immunization of cynomolgus macaques (cynos), which were subsequently challenged with a heterologous HEV strain (Mexico).
Methods : Four cynos were vaccinated intramuscularly with the HEV ORF2 DNA cassette and one animal was vaccinated with a mock DNA construct.
Results : Following vaccination anti-HEV antibodies were detected in the four HEV-DNA-vaccinated cynos, but not in the control animal. When challenged, two of the four HEV-DNA-vaccinated cynos were protected against HEV infection and had no elevated alanine aminotransferase activity, viremia, or fecal shedding. The two other DNA-vaccinated animals developed HEV infection and disease.
Conclusion : These findings demonstrate the feasibility of DNA vaccination for the protection of HEV infection and warrant further studies to explore routes other than intramuscular for induction of a stronger and efficacious immune response.
© 2002 Blackwell Publishing Asia Pty Ltd