In vitro binding of hepatitis C virus to CD81-positive and -negative human cell lines
Article first published online: 20 DEC 2002
Journal of Gastroenterology and Hepatology
Volume 18, Issue 1, pages 74–79, January 2003
How to Cite
SASAKI, M., YAMAUCHI, K., NAKANISHI, T., KAMOGAWA, Y. and HAYASHI, N. (2003), In vitro binding of hepatitis C virus to CD81-positive and -negative human cell lines. Journal of Gastroenterology and Hepatology, 18: 74–79. doi: 10.1046/j.1440-1746.2003.02925.x
- Issue published online: 20 DEC 2002
- Article first published online: 20 DEC 2002
- Accepted for publication 24 June 2002.
- hepatitis C virus;
- in vitro binding;
- reverse transcriptase–polymerase chain reaction
Aim: The aim of the present study is to study the mechanism of entry of hepatitis C virus (HCV) into human cells. We examined the in vitro binding of HCV to various human cell lines with or without CD81 expression.
Methods: We first used three cell lines, two hepatocyte-derived, huH-7 and HepG2, and one colon cancer cell line, Cw2. Among them, HepG2 did not express TAPA-1 (CD81) on their surface but two others did. They were incubated with HCV + serum for 1 h and HCV-RNA in extracted RNA obtained from these cells was examined by using both a quantitative test and reverse transcriptase–polymerase chain reaction (RT-PCR).
Results: We found that a significant amount of HCV-RNA was detected in huH-7 and HepG2 but not in Cw2. In addition, the titer of HCV-RNA in serum-incubated CD81-transfected HepG2 was similar to that of the non-transfected titer, and the binding between HCV and huH-7 was not inhibited by anti-CD81. Under the same condition, HCV-RNA tended to be detectable in serum-pulsed hepatocyte-derived cell lines, but not in the others.
Conclusion: These results suggest that while CD81, as reported, specifically binds to HCV-E2 protein, the entry of HCV into human hepatocytes might be regulated by CD81-unrelated molecule(s).
© 2003 Blackwell Publishing Asia Pty Ltd