†Mitogenomics of the commercially important fishes in Japan—I.
Complete mitochondrial DNA sequence of the Japanese sardine Sardinops melanostictus†
Article first published online: 7 JUL 2008
Volume 66, Issue 5, pages 924–932, October 2000
How to Cite
Inoue, J. G., Miya, M., Tsukamoto, K. and Nishida, M. (2000), Complete mitochondrial DNA sequence of the Japanese sardine Sardinops melanostictus. Fisheries Science, 66: 924–932. doi: 10.1046/j.1444-2906.2000.00148.x
- Issue published online: 7 JUL 2008
- Article first published online: 7 JUL 2008
- complete mitochondrial DNA sequence;
- Japanese sardine;
- Sardinops melanostictus
We determined the complete nucleotide sequence of the mitochondrial genome for a Japanese sardine, Sardinops melanostictus (Teleostei: Clupeiformes). The entire genome was purified by gene amplification using long polymerase chain reactions (PCR), and the products were subsequently used as templates for PCR with 30 sets of fish-versatile primers (including three species-specific primers) that amplify contiguous, overlapping segments of the entire genome. Direct sequencing of the PCR products demonstrated that the genome [16 881 base pairs (bp)] contained the same 37 mitochondrial structural genes (two ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) as found in other vertebrates, with the gene order identical to that in typical vertebrates. A major non-coding region between the tRNAPro and tRNAPhe genes (1200 bp) was considered as the control (D-loop) region, as it has several conservative blocks characteristic to this region.