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Complete mitochondrial DNA sequence of the Japanese sardine Sardinops melanostictus


  • †Mitogenomics of the commercially important fishes in Japan—I.

*Corresponding author: Tel: 81-3-5351-6520. Fax: 81-3-5351-6514. Email:


We determined the complete nucleotide sequence of the mitochondrial genome for a Japanese sardine, Sardinops melanostictus (Teleostei: Clupeiformes). The entire genome was purified by gene amplification using long polymerase chain reactions (PCR), and the products were subsequently used as templates for PCR with 30 sets of fish-versatile primers (including three species-specific primers) that amplify contiguous, overlapping segments of the entire genome. Direct sequencing of the PCR products demonstrated that the genome [16 881 base pairs (bp)] contained the same 37 mitochondrial structural genes (two ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) as found in other vertebrates, with the gene order identical to that in typical vertebrates. A major non-coding region between the tRNAPro and tRNAPhe genes (1200 bp) was considered as the control (D-loop) region, as it has several conservative blocks characteristic to this region.

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