Analysis of genes expressed in the mantle of oyster Crassostrea gigas

Authors

  • Hiroshi MIYAMOTO,

    Corresponding author
    1. Department of Molecular Genetics, Kinki University, Uchita, Wakayama, 649-6493,
      *Corresponding author: Tel: 81-736-77-3888. Fax: 81-736-77-4754. Email: miyamoto@gene.waka.kindai.ac.jp
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  • Masami HAMAGUCHI,

    1. Fish and Shellfish section, National Research Institute of Seto Inland Sea, Ohno, Hiroshima, 739-0452 and
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  • Kenji OKOSHI

    1. Department of Biotechnology, Senshu University of Ishinomaki, Ishinomaki, Miyagi 986-8580, Japan
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*Corresponding author: Tel: 81-736-77-3888. Fax: 81-736-77-4754. Email: miyamoto@gene.waka.kindai.ac.jp

Abstract

ABSTRACT: A total of 347 cDNA were isolated from the mantle of the oyster Crassostrea gigas by the suppression subtractive hybridization method. By northern blot analysis, we found the mRNA sequences of the several cDNA were highly expressed in the mantle. A total of 61 sequences showed close similarities to known sequences, and they were classified into seven groups: (i) protein synthesis; (ii) cytoskeleton; (iii) signal transduction; (iv) extracellular matrix; (v) metabolism; (vi) transcription factors; and (vii) others. Number 64 cDNA was more similar to the actin of Placopecten magellanicus than C. gigas, indicating that C. gigas has two isotypes of actin genes. This work is the first step to clarify functional activities of the mantle at the molecular level.

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