Wound healing and growth factor expression in T lymphocyte deficiency

  1. Matthew P. Chircop,
  2. Yan Yu,
  3. Christopher R. Berney,
  4. Jia-Lin Yang,
  5. Philip J. Crowe,
  6. William R. Walsh

Article first published online: 1 AUG 2002

DOI: 10.1046/j.1445-2197.2002.02424.x

Issue

ANZ Journal of Surgery

ANZ Journal of Surgery

Volume 72, Issue 7, pages 491–495, July 2002

Additional Information

How to Cite

Chircop, M. P., Yu, Y., Berney, C. R., Yang, J.-L., Crowe, P. J. and Walsh, W. R. (2002), Wound healing and growth factor expression in T lymphocyte deficiency. ANZ Journal of Surgery, 72: 491–495. doi: 10.1046/j.1445-2197.2002.02424.x

Author Information

  1. University of New South Wales Faculty of Medicine, Division of Surgery and Orthopaedics Research Laboratories, Prince of Wales Hospital, Sydney, Australia

* Professor W. R. Walsh, Director of Orthopaedics ­Research Laboratories, Orthopaedic Research Laboratories, University of New South Wales, Prince of Wales Hospital, Randwick, New South Wales 2031, Australia. Email: w.walsh@unsw.edu.au

Publication History

  1. Issue published online: 1 AUG 2002
  2. Article first published online: 1 AUG 2002
  3. Accepted for publication 12 February 2002.

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Keywords:

  • bFGF;
  • IGF-1;
  • mechanical properties;
  • PDGF;
  • T lymphocyte;
  • wound healing

Background:  There are many clinical situations in which immune suppression or deficiency occurs. This may lead to healing impairment. The aim of the present study was to characterize the effect of T lymphocyte deficiency on wound healing, and on ­platelet-derived growth factor (PDGF), bFGF (basic fibroblast growth factor) and insulin-like growth factor-1 (IGF-1) expression, using an animal model.

Methods:  Nude and normal 10−12-week-old male BALB/c mice were given standard dermal incisions and killed at 1, 2, 4 or 6 weeks postinjury. The pelts were harvested and the wounds cut (perpendicularly) into strips for analysis. Strips from each animal were tested mechanically or processed for histological assessment and immunohistochemical detection of PDGF, bFGF and IGF-1 and colour video image analysis. The data were assessed by analysis of variance (anova).

Results:  The interaction between species and time resulted in statistically significant differences in mechanical properties. Wound peak load was higher in nude mice at weeks 1 and 2 postinjury, but lower in nude mice at week 6 postinjury. Colour video image analysis demonstrated that the expression of bFGF and IGF-1 was greater in nude mice at week 1 postinjury (P < 0.05).

Conclusions:  The mechanical data suggest that the overall effect of the T lymphocyte system on wound healing is initially inhibitory and later stimulatory. This may be associated with corresponding differences in wound growth factor expression.

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