Initially expressed early rat embryonic GABAA receptor Cl ion channels exhibit heterogeneous channel properties

Authors

  • Ruggero Serafini,

    1. Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA,
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  • Wu Ma,

    1. Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA,
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  • Dragan Maric,

    1. Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA,
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  • Irina Maric,

    1. Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA,
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  • Fatiha Lahjouji,

    1. Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA,
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  • Werner Sieghart,

    1. Department of Biochemical Psychiatry, University Clinic for Psychiatry, Wahringer Gurtel 18-20 A 1090, Vienna, Austria
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  • Jeffery L. Barker

    1. Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA,
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Correspondence: D. Maric, Laboratory of Neurophysiology, NINDS/NIH, Bldg 36, Room 2602, Bethesda, MD 20892, USA. E-mail: Dragan@codon.nih.gov

Abstract

We have studied the earliest expression of GABA-induced Cl channels in the rat embryonic dorsal spinal cord (DSC) using in situ hybridization, immunocytochemistry, flow cytometry and electrophysiology. At embryonic day 13 (E13) cells in the dorsal region are still proliferating. In situ hybridization consistently showed transcripts encoding only three GABAa receptor subunits (α4, β1 and γ1); immunocytochemistry both in tissue sections and in acutely isolated cells in suspension demonstrated the expression of the corresponding proteins and also revealed staining for other subunits (α2, α3, β3, γ2). In patch-recordings performed in cells acutely isolated from the dorsal cord, responses to GABA were detected in 356 out of 889 cells. GABA-evoked responses, which often displayed the opening of a few channels, were mediated by Cl ions, were inhibited by bicuculline and picrotoxin, and potentiated by benzodiazepines. Taken together, these observations indicate that Cl channels likely involve GABAa type receptors. Fluctuation analysis revealed channel kinetics consisting of three exponential components (τs: ≈ 1, 9 and 90 ms) and a wide variety of inferred unitary conductance values, ranging between 4 and 40 pS. A comparison of these results with observations in other, later embryonic cell types and recombinant receptors suggests that most of the earliest E13 DSC GABAa receptors may include α3 subunit. These GABAa receptor Cl channels may be activated physiologically as both GABA synthesizing enzymes and GABA are present in the E13 dorsal cord.

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