Satellite-cell-derived nerve growth factor and neurotrophin-3 are involved in noradrenergic sprouting in the dorsal root ganglia following peripheral nerve injury in the rat

Authors

  • Xin-Fu Zhou,

    1. Department of Human Physiology and Centre for Neuroscience, Flinders University of South Australia, GPO Box 2100, Adelaide 5001, Australia
    Search for more papers by this author
  • Yan-Shen Deng,

    1. Department of Human Physiology and Centre for Neuroscience, Flinders University of South Australia, GPO Box 2100, Adelaide 5001, Australia
    Search for more papers by this author
  • Edward Chie,

    1. Department of Human Physiology and Centre for Neuroscience, Flinders University of South Australia, GPO Box 2100, Adelaide 5001, Australia
    Search for more papers by this author
  • Qing Xue,

    1. Department of Human Physiology and Centre for Neuroscience, Flinders University of South Australia, GPO Box 2100, Adelaide 5001, Australia
    Search for more papers by this author
  • Jin-Hua Zhong,

    1. Department of Human Physiology and Centre for Neuroscience, Flinders University of South Australia, GPO Box 2100, Adelaide 5001, Australia
    Search for more papers by this author
  • Elspeth M. McLachlan,

    1. Department of Human Physiology and Centre for Neuroscience, Flinders University of South Australia, GPO Box 2100, Adelaide 5001, Australia
    2. Prince of Wales Medical Research Institute, Randwick, NSW 2031, Australia
    Search for more papers by this author
  • Robert A. Rush,

    1. Department of Human Physiology and Centre for Neuroscience, Flinders University of South Australia, GPO Box 2100, Adelaide 5001, Australia
    Search for more papers by this author
  • Cory J. Xian

    1. Children Health Research Institute, Women and Children's Hospital, Adelaide, South Australia 5001, Australia
    Search for more papers by this author

X.-F. Zhou, as above. E-mail: pzxfz@cc.flinders.edu.au

Abstract

Injury to a peripheral nerve induces in the dorsal root ganglia (DRG) sprouting of sympathetic and peptidergic terminals around large-diameter sensory neurons that project in the damaged nerve. This pathological change may be implicated in the chronic pain syndromes seen in some patients with peripheral nerve injury. The mechanisms underlying the sprouting are not known. Using in situ hybridization and immunohistochemical techniques, we have now found that nerve growth factor (NGF) and neurotrophin-3 (NT3) synthesis is upregulated in satellite cells surrounding neurons in lesioned DRG as early as 48 h after nerve injury. This response lasts for at least 2 months. Quantitative analysis showed that the levels of mRNAs for NT3 and NGF increased in ipsilateral but not contralateral DRG after nerve injury. Noradrenergic sprouting around the axotomized neurons was associated with p75-immunoreactive satellite cells. Further, antibodies specific to NGF or NT3, delivered by an osmotic mini-pump to the DRG via the lesioned L5 spinal nerve, significantly reduced noradrenergic sprouting. These results implicate satellite cell-derived neurotrophins in the induction of sympathetic sprouting following peripheral nerve injury.

Ancillary