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Endogenous and exogenous nitric oxide enhance the DNA strand scission induced by tert-butylhydroperoxide in PC12 cells via peroxynitrite-dependent and independent mechanisms, respectively

Authors

  • Piero Sestili,

    1. Istituto di Farmacologia e Farmacognosia and Centro di Farmacologia Oncologica Sperimentale, Università di Urbino, Via S. Chiara, 27, 61029, Urbino (PU), Italy
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  • Emilio Clementi,

    1. Consiglio Nazionale delle Ricerche Molecular and Cellular Pharmacology Centre, and DIBIT, San Raffaele Scientific Institute, Milan, Italy
    2. Dipartimento FarmacoBiologico, Università della Calabria, Rende, Italy
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  • Andrea Guidarelli,

    1. Istituto di Farmacologia e Farmacognosia and Centro di Farmacologia Oncologica Sperimentale, Università di Urbino, Via S. Chiara, 27, 61029, Urbino (PU), Italy
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  • Clara Sciorati,

    1. Consiglio Nazionale delle Ricerche Molecular and Cellular Pharmacology Centre, and DIBIT, San Raffaele Scientific Institute, Milan, Italy
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  • Orazio Cantoni

    1. Istituto di Farmacologia e Farmacognosia and Centro di Farmacologia Oncologica Sperimentale, Università di Urbino, Via S. Chiara, 27, 61029, Urbino (PU), Italy
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: Professor Orazio Cantoni, as above.
E-mail: cantoni@fis.uniurb.it

Abstract

A short-term exposure to tert-butylhydroperoxide (tB-OOH) promoted a concentration-dependent formation of DNA single-strand breaks in PC12 cells. These events were paralleled by an increase in the cytosolic concentration of Ca2+ that was in part cleared by the mitochondria. Unlike the extent of Ca2+ mobilization and/or mitochondrial Ca2+ clearance, the DNA strand scission evoked by the hydroperoxide was markedly reduced by the nitric oxide (NO) scavenger 2-phenyl-4,4,5,5-tetramethylimidazolin-1-oxyl-3-oxide (PTIO) or by the NO synthase inhibitor N-nitro-l-arginine methylester (L-NAME). Inhibitors of electron transport (rotenone and myxothiazol), ruthenium red (RR, a polycation which inhibits the calcium uniporter of mitochondria), or peroxynitrite scavengers (Trolox and l-methionine) were as effective as PTIO or L-NAME in inhibiting the DNA-damaging response mediated by tB-OOH. Rotenone, RR or peroxynitrite scavengers did not further reduce the residual DNA cleavage observed following treatment with tB-OOH in L-NAME-supplemented cells. Exogenous NO also increased the DNA damage caused by tB-OOH in L-NAME-supplemented cells and this response was blunted by RR or by inhibitors of electron transport but was insensitive to peroxynitrite scavengers. We conclude that both endogenous and exogenous NO enhance the DNA cleavage generated by tB-OOH in PC12 cells. However, only endogenous NO set the bases for an involvement of peroxynitrite in this DNA-damaging response.

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