Present address: Service de bioénergétique, Département de Biologie Joliot Curie, CEA Saclay, Gif sur Yvette, France.
Functional characterization of a neuropeptide F-like receptor from Drosophila melanogaster
Article first published online: 22 JUL 2003
European Journal of Neuroscience
Volume 18, Issue 2, pages 227–238, July 2003
How to Cite
Feng, G., Reale, V., Chatwin, H., Kennedy, K., Venard, R., Ericsson, C., Yu, K., Evans, P. D. and Hall, L. M. (2003), Functional characterization of a neuropeptide F-like receptor from Drosophila melanogaster. European Journal of Neuroscience, 18: 227–238. doi: 10.1046/j.1460-9568.2003.02719.x
Present address: Centres & Initiatives, The Wellcome Trust, London, UK.
- Issue published online: 22 JUL 2003
- Article first published online: 22 JUL 2003
- Received 22 October 2002, revised 9 April 2003, accepted 11 April 2003
- chromosome mapping;
- G-protein-coupled receptor;
- neuropeptide F;
- receptor pharmacology;
- RNA expression;
- Xenopus oocyte expression
A cDNA clone encoding a seven-transmembrane domain, G-protein-coupled receptor (NPFR76F, also called GPCR60), has been isolated from Drosophila melanogaster. Deletion mapping showed that the gene encoding this receptor is located on the left arm of the third chromosome at position 76F. Northern blotting and whole mount in situ hybridization have shown that this receptor is expressed in a limited number of neurons in the central and peripheral nervous systems of embryos and adults. Analysis of the deduced amino acid sequence suggests that this receptor is related to vertebrate neuropeptide Y receptors. This Drosophila receptor shows 62–66% similarity and 32–34% identity to type 2 neuropeptide Y receptors cloned from a variety of vertebrate sources. Coexpression in Xenopus oocytes of NPFR76F with the promiscuous G-protein Gα16 showed that this receptor is activated by the vertebrate neuropeptide Y family to produce inward currents due to the activation of an endogenous oocyte calcium-dependent chloride current. Maximum receptor activation was achieved with short, putative Drosophila neuropeptide F peptides (Drm-sNPF-1, 2 and 2s). Neuropeptide F-like peptides in Drosophila have been implicated in a signalling system that modulates food response and social behaviour. The identification of this neuropeptide F-like receptor and its endogenous ligand by reverse pharmacology will facilitate genetic and behavioural studies of neuropeptide functions in Drosophila.