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Functional characterization of a neuropeptide F-like receptor from Drosophila melanogaster

Authors

  • Guoping Feng,

    1. Department of Neurobiology, Duke University Medical Center, Durham, NC, USA
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  • Vincenzina Reale,

    1. The Laboratory of Receptor Signalling, The Babraham Institute, Babraham, Cambridge, UK
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  • Heather Chatwin,

    1. The Laboratory of Receptor Signalling, The Babraham Institute, Babraham, Cambridge, UK
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  • Karen Kennedy,

    Corresponding author
    1. The Laboratory of Receptor Signalling, The Babraham Institute, Babraham, Cambridge, UK
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  • Renée Venard,

    1. Department of Biochemical Pharmacology, State University of New York at Buffalo, Buffalo, NY, USA
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    • Present address: Service de bioénergétique, Département de Biologie Joliot Curie, CEA Saclay, Gif sur Yvette, France.

  • Christer Ericsson,

    1. Department of Biochemical Pharmacology, State University of New York at Buffalo, Buffalo, NY, USA
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    • Present address: Cancer Center Karolinska, Karolinska Hospital, Stockholm, Sweden.

  • Kweon Yu,

    1. Functional Insect Genomics Institute, Davis, CA, USA
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    • Korean Reseach Institute of Bioscience and Biotechnology, Yusong-gu, Daejon, Korea.

  • Peter D. Evans,

    1. The Laboratory of Receptor Signalling, The Babraham Institute, Babraham, Cambridge, UK
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  • Linda M. Hall

    1. Department of Biochemical Pharmacology, State University of New York at Buffalo, Buffalo, NY, USA
    2. Functional Insect Genomics Institute, Davis, CA, USA
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    • §

      Present address: Functional Insect Genomics Institute, 1105 Kennedy Place, Suite 4, Davis, CA 95616, USA.


  • *

    Present address: Centres & Initiatives, The Wellcome Trust, London, UK.

: Dr Linda M. Hall, *Functional Insect Genomics Institute, at present address below.

Abstract

A cDNA clone encoding a seven-transmembrane domain, G-protein-coupled receptor (NPFR76F, also called GPCR60), has been isolated from Drosophila melanogaster. Deletion mapping showed that the gene encoding this receptor is located on the left arm of the third chromosome at position 76F. Northern blotting and whole mount in situ hybridization have shown that this receptor is expressed in a limited number of neurons in the central and peripheral nervous systems of embryos and adults. Analysis of the deduced amino acid sequence suggests that this receptor is related to vertebrate neuropeptide Y receptors. This Drosophila receptor shows 62–66% similarity and 32–34% identity to type 2 neuropeptide Y receptors cloned from a variety of vertebrate sources. Coexpression in Xenopus oocytes of NPFR76F with the promiscuous G-protein Gα16 showed that this receptor is activated by the vertebrate neuropeptide Y family to produce inward currents due to the activation of an endogenous oocyte calcium-dependent chloride current. Maximum receptor activation was achieved with short, putative Drosophila neuropeptide F peptides (Drm-sNPF-1, 2 and 2s). Neuropeptide F-like peptides in Drosophila have been implicated in a signalling system that modulates food response and social behaviour. The identification of this neuropeptide F-like receptor and its endogenous ligand by reverse pharmacology will facilitate genetic and behavioural studies of neuropeptide functions in Drosophila.

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