Callosal connections of dorso-lateral premotor cortex

Authors

  • B. Marconi,

    1. 1 IRCCS Santa Lucia Foundation, Rome, Italy2 Department of Human Physiology and Pharmacology, University of Rome ‘La Sapienza’, Piazzale Aldo Moro 5-00185, Rome, ItalyInstitute of 3Anatomy and 4Neurology, Catholic University, Rome, Italy
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  • 1 A. Genovesio,

    1. 1 IRCCS Santa Lucia Foundation, Rome, Italy2 Department of Human Physiology and Pharmacology, University of Rome ‘La Sapienza’, Piazzale Aldo Moro 5-00185, Rome, ItalyInstitute of 3Anatomy and 4Neurology, Catholic University, Rome, Italy
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  • 2 S. Giannetti,

    1. 1 IRCCS Santa Lucia Foundation, Rome, Italy2 Department of Human Physiology and Pharmacology, University of Rome ‘La Sapienza’, Piazzale Aldo Moro 5-00185, Rome, ItalyInstitute of 3Anatomy and 4Neurology, Catholic University, Rome, Italy
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  • 1,3 M. Molinari,

    1. 1 IRCCS Santa Lucia Foundation, Rome, Italy2 Department of Human Physiology and Pharmacology, University of Rome ‘La Sapienza’, Piazzale Aldo Moro 5-00185, Rome, ItalyInstitute of 3Anatomy and 4Neurology, Catholic University, Rome, Italy
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  • and 1,4 R. Caminiti 2

    1. 1 IRCCS Santa Lucia Foundation, Rome, Italy2 Department of Human Physiology and Pharmacology, University of Rome ‘La Sapienza’, Piazzale Aldo Moro 5-00185, Rome, ItalyInstitute of 3Anatomy and 4Neurology, Catholic University, Rome, Italy
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: Professor Roberto Caminiti, 2Department of Human Physiology and Pharmacology, as above.
E-mail: roberto.caminiti@uniroma1.it

Abstract

This study investigated the organization of the callosal connections of the two subdivisions of the monkey dorsal premotor cortex (PMd), dorso-rostral (F7) and dorso-caudal (F2). In one animal, Fast blue and Diamidino yellow were injected in F7 and F2, respectively; in a second animal, the pattern of injections was reversed. F7 and F2 receive a major callosal input from their homotopic counterpart. The heterotopic connections of F7 originate mainly from F2, with smaller contingent from pre-supplementary motor area (pre-SMA, F6), area 8 (frontal eye fields), and prefrontal cortex (area 46), while those of F2 originate from F7, with smaller contributions from ventral premotor areas (F5, F4), SMA-proper (F3), and primary motor cortex (M1). Callosal cells projecting homotopically are mostly located in layers II–III, those projecting heterotopically occupy layers II–III and V–VI. A spectral analysis was used to characterize the spatial fluctuations of the distribution of callosal neurons, in both F7 and F2, as well as in adjacent cortical areas. The results revealed two main periodic components. The first, in the domain of the low spatial frequencies, corresponds to periodicities of cell density with peak-to-peak distances of approximately 10 mm, and suggests an arrangement of callosal cells in the form of 5-mm wide bands. The second corresponds to periodicities of approximately 2 mm, and probably reflects a 1-mm columnar-like arrangement. Coherency and phase analyses showed that, although similar in their spatial arrangements, callosal cells projecting to dorsal premotor areas are segregated in the tangential cortical domain.

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