Direct and indirect estimates of the selfing rate in small and large individuals of the bumblebee pollinated Cynoglossum officinale L (Boraginaceae)

Authors

  • K. Vrieling,

    1. Laboratoire de Génétique et Évolution des Populations Végétales. UPRESA CURS 901C. Université de Lille. Bâtiment SN2. F-59655 Villeneuve d’Ascq CEDEX France. And, Institute of Evolutionary and Ecological Sciences, Van der Klaauw Laboratory, PO Box 9516, 2300 RA Leiden, The Netherlands.,
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  • P. Saumitou- Laprade,

    1. Laboratoire de Génétique et Évolution des Populations Végétales. UPRESA CURS 901C. Université de Lille. Bâtiment SN2. F-59655 Villeneuve d’Ascq CEDEX France.,
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  • J. Cuguen,

    1. Laboratoire de Génétique et Évolution des Populations Végétales. UPRESA CURS 901C. Université de Lille. Bâtiment SN2. F-59655 Villeneuve d’Ascq CEDEX France.,
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  • H. Van Dijk,

    1. Laboratoire de Génétique et Évolution des Populations Végétales. UPRESA CURS 901C. Université de Lille. Bâtiment SN2. F-59655 Villeneuve d’Ascq CEDEX France.,
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  • T.J. De Jong,

    1. Institute of Evolutionary and Ecological Sciences, Van der Klaauw Laboratory, PO Box 9516, 2300 RA Leiden, The Netherlands.,
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  • P.G.L. Klinkhamer

    1. Institute of Evolutionary and Ecological Sciences, Van der Klaauw Laboratory, PO Box 9516, 2300 RA Leiden, The Netherlands.
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Klaas Vrieling Institute of Evolutionary and Ecological Sciences.E-mail: Vrieling@RULSFB.Leidenuniv.nL

Abstract

We measured the relationship between selfing rates and flower number in an experimental population of bumblebee pollinated Cynoglossum officinale, with plants differing in flower number. Results were compared with the prediction of a model based on pollen dynamics and pollinator behaviour. The selfing rate, as measured by multilocus oligonucleotide DNA fingerprinting, increased with flower number and ranged from 0% to 70%. Flowers on large plants received an equal number of visits from bumblebees as flowers on small plants. On large plants more flowers in a row were visited, inducing geitonogamy. The overall relationship between selfing rate and number of flowers can be explained by pollen dynamics and pollinator behaviour without invoking postpollination processes such as differential pollen tube growth and abortion.

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